Rabbit Anti-KDM1A Recombinant Antibody (clone R08-3J5)
CAT#: VS3-FY1755
This R08-3J5 recombinant monoclonal antibody specifically attaches to human KDM1A. It cross-reacts with hamster, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This KDM1A antibody has been rigorously validated and is widely used in western blot analysis of KDM1/LSD1 in Hela, CHO-K1, C6, Jurkat lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53660/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/if_selected.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004487-KDM1A
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WB
Figure 1 Anti-KDM1A Antibody (VS3-FY1755) in WB.
The KDM1/LSD1 expression in Hela, CHO-K1, C6, Jurkat lysates were detected using the anti-KDM1/LSD1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human KDM1/LSD1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster, Rat KDM1A
- Species Reactivity
- Human, Hamster, Rat
- Clone
- R08-3J5
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 93 kDa, while the observed molecular weight stands at 110 kDa.
- Related Disease
- Cleft Palate, Psychomotor Retardation, And Distinctive Facial Features and Neuroblastoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Lysine Demethylase 1A; Flavin-Containing Amine Oxidase Domain-Containing Protein 2; Amine Oxidase (Flavin Containing) Domain 2; BRAF35-HDAC Complex Protein BHC110; AOF2; KDM1; LSD1; FAD-Binding Protein BRAF35-HDAC Complex, 110 KDa Subunit; Lysine-Specific Histone Demethylase 1A
- Gene ID
- 23028
- UniProt ID
- O60341
- Sequence Similarities
- Belongs to the flavin monoamine oxidase family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Polyubiquitinated by JADE2; which leads to its proteasomal degradation (PubMed:25018020).
Deubiquitinated by USP38; preventing it from degradation by the 26S proteasome (PubMed:30497519).
- Protein Refseq
- XP_005245843.1; XP_006710536.1; XP_016856205.1; XP_047305633.1; XP_054191226.1
- Function
- Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me.
May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.
Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1924 | Hi-Affi™ Recombinant Rabbit Anti-KDM1A Monoclonal Antibody (DS1924AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
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| ZG-0784J | Rabbit Anti-KDM1A Recombinant Antibody (clone 1G7) | ELISA, WB, IHC | Rabbit IgG |
| VS3-XY960 | Mouse Anti-KDM1A Recombinant Antibody (clone 3G1) | WB, IP, IF | Mouse IgG1 |
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| VS7-0425-WR189 | Mouse Anti-KDM1A Recombinant Antibody (clone 3F9D2) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR320 | Mouse Anti-KDM1A Monoclonal Antibody (clone 1B2E5) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3746 | Anti-KDM1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3747 | Anti-Mouse KDM1A Immunohistochemistry Kit | IHC |
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