Human Anti-KDM4A Recombinant Antibody (clone 2506W1353)
CAT#: VS-0625-FY1458
This recombinant antibody is a human IgG1 antibody, generated using recombinant antibody (rAb) phage display. Its specificity and affinity for the KDM4A protein have been confirmed under non-denaturing conditions. While specific applications are not detailed, this antibody is a robust tool for recognizing KDM4A. The antibody targets an epitope spanning amino acids 2-359 of the KDM4A protein, making it valuable for studying KDM4A in its native conformation or for developing custom assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/75_D3_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/74_D3_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_B_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000066135-KDM4A
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- The antibody reacts with human KDM4A.
- Species Reactivity
- Human
- Clone
- 2506W1353
- Applications
- ELISA, Immunofluorescence, Immunoprecipitation Mass Spectrometry
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (over 6 months). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- Lysine Demethylase 4A; JmjC Domain-Containing Histone Demethylation Protein 3A; Jumonji C Domain-Containing Histone Demethylase 3A; Jumonji Domain-Containing Protein 2A; Lysine (K)-Specific Demethylase 4A; Jumonji Domain Containing 2A; Tudor Domain Containing 14A; Jumonji Domain Containing 2; JMJD2A
- Gene ID
- 9682
- UniProt ID
- O75164
- Long Name
- Lysine Demethylase 4A
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KDM4A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-366 | Recombinant Human Anti-Human KDM4A Antibody Fab Fragment, clone D266 | IP, IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0521J | Mouse Anti-KDM4A Recombinant Antibody (ZG-0521J) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-WK56 | Mouse Anti-KDM4A Recombinant Antibody (clone E7-H1) | WB, ELISA | Mouse IgG1 |
| VS3-WK137 | Mouse Anti-KDM4A Recombinant Antibody (clone G4-E10) | WB, ELISA | Mouse IgG1 |
| VS7-0425-WR200 | Mouse Anti-KDM4A Recombinant Antibody (clone 6E10G4) | ICC, FC | Mouse IgG1 |
| VS7-0425-WR201 | Mouse Anti-KDM4A Monoclonal Antibody (VS7-0425-WR201) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3752 | Anti-KDM4A Immunohistochemistry Kit | IHC |
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