Mouse Anti-KDM5A Recombinant Antibody (clone 0725W71)
CAT#: VS-0725-FY238
This recombinant antibody, clone 0725W71, targets full-length, recombinant human KDM5A. It is validated for Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) and Western Blot (WB). KDM5A, a member of the JARID1 histone demethylase family, plays a role in gene regulation by specifically demethylating lysine 4 of histone H3. This antibody is a valuable tool for studying epigenetic mechanisms, transcriptional regulation of Hox genes and cytokines, and its potential role in tumor progression.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6201/43_F7_2_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000073614-KDM5A
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Specifications
- Immunogen
- Full-length, recombinant human KDM5A.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human KDM5A
- Species Reactivity
- Human
- Clone
- 0725W71
- Applications
- Chromatin Immunoprecipitation-Sequencing, Western Blot
- Related Disease
- Retinoblastoma
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 196 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation-Sequencing: 4 µg per Chromatin Immunoprecipitation
Western Blot: 0.5-2 µg/mL
Target
- Alternative Names
- Lysine Demethylase 5A; Jumonji/ARID Domain-Containing Protein 1A; Lysine (K)-Specific Demethylase 5A; Retinoblastoma-Binding Protein 2; Histone Demethylase JARID1A; RBBP-2; RBBP2; RBP2; Jumonji, AT Rich Interactive Domain 1A (RBBP2-Like)
- Gene ID
- 5927
- UniProt ID
- P29375
- Long Name
- Lysine Demethylase 5A
- Cellular Localization
- Nucleus, nucleolus
- Post Translation Modifications
- Ubiquitination at Lys347, Lys425, Lys733, and Lys1275
Modification sites
Glycosylation from GlyGen (P29375) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001036068.1
- Function
- Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code.
Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'.
Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'.
Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1432MZ | Recombinant Mouse Anti-Human KDM5A Antibody (clone 29F9) | WB | Mouse antibody |
| MRO-0896-CN | Recombinant Rabbit Anti-KDM5A Monoclonal Antibody (JB36-60) | WB, IF, FC | Rabbit IgG |
| VS3-WK289 | Rabbit Anti-KDM5A Recombinant Antibody (clone JB36-60) | WB, ELISA | Rabbit IgG |
| VS3-CJ50 | Mouse Anti-KDM5A Recombinant Antibody (VS3-CJ50) | WB, ELISA | Mouse IgG |
| VS3-XY3406 | Rabbit Anti-KDM5A Recombinant Antibody (clone R02-3F8) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1925 | Hi-Affi™ Recombinant Rabbit Anti-KDM5A Monoclonal Antibody (DS1925AB) | FC, WB | IgG |
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