Mouse Anti-KDM6A Recombinant Antibody (clone 5C2A9) (CAT#: VS7-0425-WR205)
This product is a mouse IgG2a antibody that binds to human KDM6A. The antibody 5C2A9 recognizes specific amino acid domain of human KDM6A (AA: 1252-1401). It can be used for FC assay.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
Subcellular Location
RNA Expression
Figure 1 Bone marrow
(High expression in bone marrow)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_B_4_4_val_selected.jpg
(High expression in bone marrow)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_B_4_4_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_B_8_5.jpg
Figure 4 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_9_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_9_3.jpg
Figure 5 Placenta
(Cytotrophoblasts Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity: 75%-25% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_3_7.jpg
(Cytotrophoblasts Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity: 75%-25% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_3_7.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_8_8.jpg
Figure 2 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/568/1858_B11_32_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/568/1858_B11_32_selected.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147050-KDM6A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147050-KDM6A
Specifications
- Immunogen
- Recombinant human KDM6A (AA: 1252-1401) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human KDM6A
- Species Reactivity
- Human
- Clone
- 5C2A9
- Molecule Class
- Demethylase
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Kabuki syndrome 2 (KABUK2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KDM6A; BA386N14.2; EC 1.14.11; Ubiquitously-Transcribed X Chromosome Tetratricopeptide Repeat Protein; Ubiquitously-Transcribed TPR Protein On The X Chromosome; Ubiquitously Transcribed Tetratricopeptide Repeat, X Chromosome; Ubiquitously-Transcribed TPR
- Gene ID
- 7403
- UniProt ID
- O15550
- Long Name
- Lysine demethylase 6A
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Methylation; Phosphoprotein
- Protein Refseq
- NP_001278344.1
- Function
- Chromatin regulator; Dioxygenase; Oxidoreductase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3996z | Mouse Anti-KDM6A Recombinant Antibody (clone 26G12) | WB, ELISA, FC, ICC, IHC | Mouse IgG2a |
| MOB-0105CT | Recombinant Mouse anti-Human KDM6A Monoclonal antibody (4B3) | ELISA, IP, WB | |
| VS3-CJ42 | Mouse Anti-KDM6A Recombinant Antibody (VS3-CJ42) | WB, ELISA | Mouse IgG |
| VS7-0425-WR206 | Mouse Anti-KDM6A Recombinant Antibody (clone 6E2D6) | WB | Mouse IgG2a |
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