Mouse Anti-KPNA2 Recombinant Antibody (clone 1E8B7) (CAT#: VS7-0425-WR247)
This product is a mouse IgG1 antibody that binds to human KPNA2. The antibody 1E8B7 recognizes specific amino acid domain of human KPNA2 (AA: 1-530). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with mouse.
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Custom Production
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41270/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41270/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/945_C8_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/945_C8_3_selected.jpg
Figure 3 Testis
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/34903_A_4_6.jpg
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/34903_A_4_6.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/34903_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/34903_A_8_8.jpg
Figure 5 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/34903_B_7_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/34903_B_7_5.jpg
Figure 6 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/34903_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15460/34903_A_8_4.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182481-KPNA2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182481-KPNA2
Specifications
- Immunogen
- Recombinant human KPNA2 (AA: 1-530) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KPNA2
- Species Reactivity
- Human, Mouse
- Clone
- 1E8B7
- Molecule Class
- Importin Alpha Family Of Proteins
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KPNA2; SRP1-Alpha; Importin Alpha 1; RAG Cohort Protein 1; Importin Subunit Alpha-2; SRP1alpha; IPOA1; Importin Alpha 2; SRP1; RCH1
- Gene ID
- 3838
- UniProt ID
- P52292
- Long Name
- Karyopherin subunit alpha 2
- Cellular Localization
- Cytoplasm; Endoplasmic reticulum; Golgi apparatus; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001307540.1
- Function
- Host-virus interaction; Protein transport; Transport
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3100z | Mouse Anti-KPNA2 Recombinant Antibody (clone 4B8) | WB, ELISA, FC, ICC, IF, IHC, ICC, IF | Mouse IgG1 |
| MRO-0906-CN | Recombinant Rabbit Anti-KPNA2 Monoclonal Antibody (JM90-33) | WB, FC | Rabbit IgG |
| VS1-YJ116 | Rat Anti-KPNA2 Recombinant Antibody (clone 2G7) | WB | Rat IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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