CytoStream™ Rabbit Anti-KRT13 Polyclonal Antibody (VS13-YC632) (CAT#: VS13-YC632)
The CytoStream™ Rabbit Anti-KRT13 Polyclonal Antibody is specific for human, mouse, rat and Cow KRT13. KRT13, a keratin gene family member, affects epithelia structural integrity, expressed with keratin 4 in suprabasal non-cornified layers. Mutations link to autosomal dominant White Sponge Nevus disorder, clustered in chromosome 17q21.2. The anti-KRT13 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 μg per 1x 10⁶ cells).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30877/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30877/if_selected.jpg
Figure 2 High expression in esophagus
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30877/142302_B_4_1_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30877/142302_B_4_1_val_selected.jpg
Figure 3 Low expression in skeletal muscle
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30877/142302_B_1_6_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30877/142302_B_1_6_val_selected.jpg
Figure 4 Esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30877/142302_B_4_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30877/142302_B_4_1.jpg
Figure 5 RNA cell line category: Group enriched (A-431, HaCaT)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171401-KRT13
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171401-KRT13
Specifications
- Immunogen
- Recombinant human cytokeratins conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat and Cow KRT13
- Species Reactivity
- Human, Mouse, Rat, Cow, Chicken (Predicted), Dog (Predicted), Pig (Predicted), Horse (Predicted), Rabbit (Predicted)
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/2000
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 μg per 1x 10⁶ cells
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- Keratin 13; Keratin, Type I Cytoskeletal 13; Keratin 13, Type I; Cytokeratin 13; CK-13; K13;
- Gene ID
- 3860
- UniProt ID
- P13646
- Long Name
- Keratin 13
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Intermediate filament; Keratin
- Protein Refseq
- NP_002265.2; NP_705694.2
- Function
- Type 1 keratin (Probable). Maintains postnatal tongue mucosal cell homeostasis and tissue organization in response to mechanical stress, potentially via regulation of the G1/S phase cyclins CCNE1 and CCNE2 (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0537CT | Recombinant Mouse anti-Human KRT13 Monoclonal antibody (1C7) | IHC-Fr, IHC-P | |
| MRO-0435-CN | Recombinant Rabbit Anti-KRT13 Monoclonal Antibody (CBACN-175) | WB, IF, IHC | Rabbit IgG |
| MRO-1804-CN | Rabbit Anti-KRT13 Polyclonal Antibody (MRO-1804-CN) | WB, IHC, IF | Rabbit IgG |
| ZG-0800F | Mouse Anti-KRT13 Recombinant Antibody (clone DE-K13) | WB, IHC | Mouse IgG2a |
| VS4-CJ2 | Recombinant Mouse Anti-KRT13 Antibody (VS4-CJ2) | ELISA, IHC | Mouse IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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