Mouse Anti-KRT2 Recombinant Antibody (clone 1A2B5) (CAT#: VS7-0425-WR262)
This product is a mouse IgG2a antibody that binds to human KRT2. The antibody 1A2B5 recognizes specific amino acid domain of human KRT2 (AA: 177-490). It can be used for WB, IHC, ICC, and FC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6299/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6299/if_selected.jpg
Figure 2 High expression in skin 1.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_B_7_1_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_B_7_1_selected.jpg
Figure 3 Low expression in colon.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_A_7_3_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_A_7_3_val_selected.jpg
Figure 4 Skin
(Cells in basal layer Staining: Low Intensity: Weak Quantity:>75% Cells in corneal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_B_7_1.jpg
(Cells in basal layer Staining: Low Intensity: Weak Quantity:>75% Cells in corneal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_B_7_1.jpg
Figure 5 RNA cell line category: Cell line enhanced (HEL)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172867-KRT2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172867-KRT2
Specifications
- Immunogen
- Recombinant human KRT2 (AA: 177-490) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human KRT2
- Species Reactivity
- Human
- Clone
- 1A2B5
- Molecule Class
- Type II Keratin Family
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Ichthyosis bullosa of Siemens (IBS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- K2e; KRTE; CK-2e; KRT2A; KRT2E
- Gene ID
- 3849
- UniProt ID
- P35908
- Long Name
- Keratin 2
- Cellular Localization
- Cytoplasm; Intermediate filament; Keratin
- Post Translation Modifications
- Methylation; Phosphoprotein
- Protein Refseq
- NP_000414.2
- Function
- Probably contributes to terminal cornification (PubMed:1380918).; Associated with keratinocyte activation, proliferation and keratinization (PubMed:12598329).; Required for maintenance of corneocytes and keratin filaments in suprabasal keratinocytes in the epidermis of the ear, potentially via moderation of expression and localization of keratins and their partner proteins (By similarity).; Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2375CT | Recombinant Mouse anti-Human KRT2 Monoclonal antibody (TCG6) | WB, IHC-P, IHC-Fr | |
| MRO-0445-CN | Recombinant Rabbit Anti-KRT2 Monoclonal Antibody (CBACN-183) | WB, IHC | Rabbit IgG |
| VS3-CJ912 | Rabbit Anti-KRT2 Recombinant Antibody (VS3-CJ912) | WB, IHC | Rabbit IgG |
| VS3-FY2757 | Mouse Anti-KRT2 Recombinant Antibody (VS3-FY2757) | WB, IHC, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR263 | Mouse Anti-KRT2 Recombinant Antibody (clone 8A1E1) | IHC, ICC | Mouse IgG2a |
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