Rabbit Anti-KRT2 Recombinant Antibody (VS3-CJ912)
CAT#: VS3-CJ912
This product is a rabbit antibody that recognizes human KRT2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6299/if_selected.jpg
Normal Tissue
Figure 2 High expression in skin 1.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_B_7_1_selected.jpg
Normal Tissue
Figure 3 Low expression in colon.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_A_7_3_val_selected.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: Low Intensity: Weak Quantity:>75% Cells in corneal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HEL)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172867-KRT2
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KRT2
- Species Reactivity
- Human
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistochemistry.
Target
- Alternative Names
- K2e; KRTE; CK-2e; KRT2A; KRT2E
- Gene ID
- 3849
- UniProt ID
- P35908
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin
- Protein Refseq
- NP_000414.2
- Function
- Probably contributes to terminal cornification (PubMed:1380918).
Associated with keratinocyte activation, proliferation and keratinization (PubMed:12598329).
Required for maintenance of corneocytes and keratin filaments in suprabasal keratinocytes in the epidermis of the ear, potentially via moderation of expression and localization of keratins and their partner proteins (By similarity).
Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KRT2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2375CT | Recombinant Mouse anti-Human KRT2 Monoclonal antibody (TCG6) | WB, IHC-P, IHC-Fr | |
| MRO-0445-CN | Recombinant Rabbit Anti-KRT2 Monoclonal Antibody (CBACN-183) | WB, IHC | Rabbit IgG |
| VS3-FY2757 | Mouse Anti-KRT2 Recombinant Antibody (VS3-FY2757) | WB, IHC, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR262 | Mouse Anti-KRT2 Recombinant Antibody (clone 1A2B5) | WB, IHC, ICC, FC | Mouse IgG2a |
| VS7-0425-WR263 | Mouse Anti-KRT2 Recombinant Antibody (clone 8A1E1) | IHC, ICC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1984 | Hi-Affi™ Recombinant Rabbit Anti-KRT2 Monoclonal Antibody (DS1984AB) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1215 | Anti-KRT2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3865 | Anti-Human KRT2 Immunohistochemistry Kit | IHC |
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