Mouse Anti-KRT2 Recombinant Antibody (VS3-FY2757)
CAT#: VS3-FY2757
This anti-KRT2 product is a mouse recombinant monoclonal antibody that has been carefully developed to target KRT2. The calculated molecular weight of the antigen is 65.4 kDa. It cross-reacts with human. This monoclonal antibody is potential for conducting research on Ichthyosis Bullosa Of Siemens and Epidermolytic Hyperkeratosis 1. This monoclonal antibody can be used in Western Blot, Immunohistochemistry, Immunocytochemistry, Flow cytometry, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6299/if_selected.jpg
Normal Tissue
Figure 2 High expression in skin 1.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_B_7_1_selected.jpg
Normal Tissue
Figure 3 Low expression in colon.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_A_7_3_val_selected.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: Low Intensity: Weak Quantity:>75% Cells in corneal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37321/140905_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HEL)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172867-KRT2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Keratin 2 of human
- Species Reactivity
- Human
- Applications
- WB, IHC, ICC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 65.4 kDa.
- Related Disease
- Ichthyosis Bullosa Of Siemens and Epidermolytic Hyperkeratosis 1
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Immunocytochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Keratin 2; Epithelial Keratin-2e; Keratin-2 Epidermis; Type-II Keratin Kb2; Keratin 2, Type II; Cytokeratin-2e; Keratin-2e; KRT2A
- Gene ID
- 3849
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Modification sites at PhosphoSitePlus
Modification sites at neXtProt
Glycosylation from GlyGen 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- NP_000414.2
- Function
- Associated with keratinocyte activation, proliferation and keratinization (PubMed:12598329).
Required for maintenance of corneocytes and keratin filaments in suprabasal keratinocytes in the epidermis of the ear, potentially via moderation of expression and localization of keratins and their partner proteins (By similarity).
Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KRT2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2375CT | Recombinant Mouse anti-Human KRT2 Monoclonal antibody (TCG6) | WB, IHC-P, IHC-Fr | |
| MRO-0445-CN | Recombinant Rabbit Anti-KRT2 Monoclonal Antibody (CBACN-183) | WB, IHC | Rabbit IgG |
| VS3-CJ912 | Rabbit Anti-KRT2 Recombinant Antibody (VS3-CJ912) | WB, IHC | Rabbit IgG |
| VS7-0425-WR262 | Mouse Anti-KRT2 Recombinant Antibody (clone 1A2B5) | WB, IHC, ICC, FC | Mouse IgG2a |
| VS7-0425-WR263 | Mouse Anti-KRT2 Recombinant Antibody (clone 8A1E1) | IHC, ICC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1984 | Hi-Affi™ Recombinant Rabbit Anti-KRT2 Monoclonal Antibody (DS1984AB) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1215 | Anti-KRT2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3865 | Anti-Human KRT2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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