Mouse Anti-KRT5 Monoclonal Antibody (VS7-0425-WR266)
CAT#: VS7-0425-WR266
This product is a mouse IgG antibody that binds to human KRT5. The antibody can be used for WB, IHC-P, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line hTCEpi
Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/1790_D3_5_selected.jpg
Normal Tissue
Figure 2 High expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_2_6_val_selected.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: High Intensity: Strong Quantity:>75% Hair follicles Staining: High Intensity: Strong Quantity: 75%-25% Sebaceous glands Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HaCaT, HBEC3-KT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186081-KRT5
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Specifications
- Immunogen
- Recombinant human KRT5 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KRT5
- Species Reactivity
- Human
- Molecule Class
- Type II Cytokeratins
- Applications
- WB, IHC-P, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Epidermolysis bullosa simplex 2A, generalized severe (EBS2A); Epidermolysis bullosa simplex 2B, generalized intermediate (EBS2B); Epidermolysis bullosa simplex 2C, localized (EBS2C); Epidermolysis bullosa simplex 2D, generalized, intermediate or severe, autosomal recessive (EBS2D); Epidermolysis bullosa simplex 2E, with migratory circinate erythema (EBS2E); Epidermolysis bullosa simplex 2F, with mottled pigmentation (EBS2F); Dowling-Degos disease 1 (DDD1)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Immunohistochemistry: 1:200 - 1:1000
Target
- Alternative Names
- KRT5; KRT5A; Keratin 5; EBS2; Cytokeratin-5; CK5; DDD1; K5; Keratin 5 (Epidermolysis Bullosa Simplex, Dowling-Meara/Kobner/Weber-Cockayne Types); 58 Kda Cytokeratin; Keratin, Type II Cytoskeletal 5; Keratin-5; Keratin 5, Type II; Type-II Keratin Kb5; Epid
- Gene ID
- 3852
- UniProt ID
- P13647
- Long Name
- Keratin 5
- Cellular Localization
- Cytoplasm; Intermediate filament; Keratin
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_000415.2
- Function
- Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity).; Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KRT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1990 | Hi-Affi™ Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (DS1990AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0448-CN | Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (SY88-19) | WB, IHC | Rabbit IgG |
| MRO-0449-CN | Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (CBACN-186) | WB, IF, IHC | Rabbit IgG |
| VS3-QX655 | Mouse Anti-KRT5 Recombinant Antibody (clone 7H5-B5-B6-G8) | WB | Mouse IgG1 |
| VS3-QX656 | Mouse Anti-KRT5 Recombinant Antibody (clone 7B4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX657 | Mouse Anti-KRT5 Recombinant Antibody (clone 6G5-B1-B9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1518 | AbPlus™ Anti-KRT5 Magnetic Beads (VS-0724-YC1518) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC636 | CytoStream™ Rabbit Anti-KRT5 Recombinant Antibody (VS13-YC636) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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