Mouse Anti-KRT5 Recombinant Antibody (clone AbM58019-7H4)
CAT#: VS11-QX95
This product is a recombinant mouse antibody that recognizes KRT5. This antibody can be used for the following applications: ELISA, IHC. This antibody has the ability to bind to both human cytokeratin 5 and human cytokeratin 6.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line hTCEpi
Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/1790_D3_5_selected.jpg
Normal Tissue
Figure 2 High expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_2_6_val_selected.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: High Intensity: Strong Quantity:>75% Hair follicles Staining: High Intensity: Strong Quantity: 75%-25% Sebaceous glands Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HaCaT, HBEC3-KT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186081-KRT5
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Specifications
- Immunogen
- Recombinant protein comprising amino acids 488-590 of cytokeratin 5
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Human KRT5
- Species Reactivity
- Human
- Clone
- AbM58019-7H4
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- Keratin 5; Keratin 5 (Epidermolysis Bullosa Simplex, Dowling-Meara/Kobner/Weber-Cockayne Types); Epidermolysis Bullosa Simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; Type-II Keratin Kb5; Keratin 5, Type II; Cytokeratin-5; CK-5; K5; Keratin, Type II Cytoskeletal 5
- Gene ID
- 3852
- UniProt ID
- P13647
- Long Name
- Keratin, type II cytoskeletal 5
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Intermediate filaments
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KRT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1990 | Hi-Affi™ Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (DS1990AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX655 | Mouse Anti-KRT5 Recombinant Antibody (clone 7H5-B5-B6-G8) | WB | Mouse IgG1 |
| VS3-QX656 | Mouse Anti-KRT5 Recombinant Antibody (clone 7B4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX657 | Mouse Anti-KRT5 Recombinant Antibody (clone 6G5-B1-B9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX658 | Mouse Anti-KRT5 Recombinant Antibody (clone 6D-4D) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS3-QX659 | Mouse Anti-KRT5 Recombinant Antibody (clone 7H8) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1518 | AbPlus™ Anti-KRT5 Magnetic Beads (VS-0724-YC1518) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC636 | CytoStream™ Rabbit Anti-KRT5 Recombinant Antibody (VS13-YC636) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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