Mouse Anti-KRT5 Recombinant Antibody (clone ABT-CK5) (CAT#: VS3-CJ635)
This product is a mouse antibody that recognizes human KRT5.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line hTCEpi
(Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/1790_D3_5_selected.jpg
(Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/1790_D3_5_selected.jpg
Figure 2 High expression in skin 1
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1_selected.jpg
Figure 3 Low expression in skeletal muscle
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_2_6_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_2_6_val_selected.jpg
Figure 4 Skin
(Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: High Intensity: Strong Quantity:>75% Hair follicles Staining: High Intensity: Strong Quantity: 75%-25% Sebaceous glands Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1.jpg
(Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: High Intensity: Strong Quantity:>75% Hair follicles Staining: High Intensity: Strong Quantity: 75%-25% Sebaceous glands Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1.jpg
Figure 5 RNA cell line category: Group enriched (HaCaT, HBEC3-KT, hTCEpi)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186081-KRT5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186081-KRT5
Specifications
- Immunogen
- Synthesized peptide derived from human Cytokeratin 5
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Human KRT5
- Species Reactivity
- Human
- Clone
- ABT-CK5
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- K5; CK5; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; KRT5A
- Gene ID
- 3852
- UniProt ID
- P13647
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Intermediate filament, Keratin
- Post Translation Modifications
- Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle (By similarity).
Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis (By similarity).
CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury (By similarity).
- Protein Refseq
- NP_000415.2
- Function
- Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1990 | Hi-Affi™ Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (DS1990AB) | IHC-P | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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