Mouse Anti-KRT5 Recombinant Antibody (ZG-0250J)
CAT#: ZG-0250J
This product is a mouse antibody that recognizes KRT5.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-KRT5 Recombinant Antibody (ZG-0250J) in IHC
Immunohistochemical analysis of paraffin-embedded Squamous cell lung carcinoma. 1. Antibody was diluted at 1:200 (4°C overnight). 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
IHC
Figure 2 Mouse Anti-KRT5 Recombinant Antibody (ZG-0250J) in IHC
Immunohistochemical analysis of paraffin-embedded human Cervical squamous cell carcinoma-High magnification. 1.Cytokeratin 5 Antibody was diluted at 1:200 (4°C overnight). 2, EDTA pH 8.0 was used for antigen retrieva
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Subcellular Location
Figure 1 IF staining of human cell line hTCEpi
Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/1790_D3_5_selected.jpg
Normal Tissue
Figure 2 High expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_2_6_val_selected.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: High Intensity: Strong Quantity:>75% Hair follicles Staining: High Intensity: Strong Quantity: 75%-25% Sebaceous glands Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HaCaT, HBEC3-KT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186081-KRT5
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Specifications
- Immunogen
- Synthesized peptide derived from human Cytokeratin 5
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KRT5
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
- Alternative Names
- K5; CK5; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; KRT5A; keratin, type II cytoskeletal 5; 58 kda cytokeratin; CK-5; cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; type-II keratin Kb5
- Gene ID
- 3852
- UniProt ID
- P13647
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "KRT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1990 | Hi-Affi™ Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (DS1990AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0459F | Mouse Anti-KRT5 Recombinant Antibody (ZG-0459F) | IHC, IF, ELISA | Mouse IgG |
| ZG-0460F | Mouse Anti-KRT5 Recombinant Antibody (ZG-0460F) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0467F | Mouse Anti-KRT5 Recombinant Antibody (ZG-0467F) | WB, ELISA | Mouse IgG |
| ZG-0468F | Mouse Anti-KRT5 Recombinant Antibody (ZG-0468F) | WB, IHC, ELISA | Mouse IgG |
| VS3-QX655 | Mouse Anti-KRT5 Recombinant Antibody (clone 7H5-B5-B6-G8) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1518 | AbPlus™ Anti-KRT5 Magnetic Beads (VS-0724-YC1518) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC636 | CytoStream™ Rabbit Anti-KRT5 Recombinant Antibody (VS13-YC636) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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