Rabbit Anti-LAMP2 Recombinant Antibody (VS3-CJ628)
CAT#: VS3-CJ628
This product is a rabbit antibody that recognizes human, mouse, and rat LAMP2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/598_H2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5272/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005893-LAMP2
❮
❯
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat LAMP2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- DND; LAMPB; CD107b; LAMP-2; LGP-96; LGP110
- Gene ID
- 3920
- UniProt ID
- P13473
- Sequence Similarities
- Belongs to the LAMP family.
- Cellular Localization
- Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane
- Post Translation Modifications
- O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
- Protein Refseq
- NP_001116078.1; NP_002285.1; NP_054701.1
- Function
- Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032).
Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125).
Plays a role in lysosomal protein degradation in response to starvation (By similarity).
Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032).
Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032).
Required for normal degradation of the contents of autophagosomes (PubMed:27628032).
Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits (PubMed:20518820).
Is not required for efficient MHCII-mediated presentation of endogenous antigens (PubMed:20518820).
Isoform LAMP-2C
Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.
(Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
| MOB-0951CT | Recombinant Mouse anti-Human LAMP2 Monoclonal antibody (I5C5) | FC, IHC-Fr | |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0325-XY1225 | Anti-LAMP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3941 | Anti-Mouse LAMP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3940 | Anti-Human LAMP2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC645 | CytoStream™ Mouse Anti-LAMP2 Recombinant Antibody (VS13-YC645) | WB, ICC, IF, IHC-P, FC | Mouse IgG1 |
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