Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (CAT#: MOB-0086F)
This product is a mouse antibody that recognizes LC3A.
Specific Inquiry
IF
Figure 1 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in IF
Immunofluorescence analysis of Hela cells. 1. CREB-1 polyclonal antibody (red) is diluted 1:200 (overnight at 4°C). The LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 594 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor 488 is diluted 1:1000 (room temperature, 50 minutes).
WB
Figure 2 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in WB
From 1) Hela Cell Lysate, 2) 3T3 CellLysate, 3) Western blot analysis of lysates of rat brain cells, (green) primary antibody diluted 1:1000, overnight at 4°C, Dylight 800 secondary antibody (at 1: Diluted at 10000 for 1 hour at 37°C. (red) Actin β polyclonal antibody was diluted at 1:5000 as a loading control, overnight at 4°C, and Dylight 680 secondary antibody (diluted at 1:10000, at 37°C for 1 hour.
IHC
Figure 3 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in IHC
Immunohistochemical analysis of paraffin-embedded human uterine tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.
IHC
Figure 4 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.
IF
Figure 5 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in IF
Immunofluorescence analysis of human lung cancer tissue. 1. LC3A mouse monoclonal antibody (5G10) (red) is diluted 1:200 (4°C, overnight). 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Picture B: DAPI. Picture C: The merger of A+B.
WB
Figure 6 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in WB
Western blot analysis was performed on 1) Hela cell lysate, 2) 3T3 cell lysate, and 3) rat brain tissue lysate using LC3A Mouse mAb diluted 1:1000.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human LC3A
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 5G10
- Applications
- WB, IF, IHC-P
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunofluorescence, Immunohistologyp-Paraffin Sections.
Target
- Alternative Names
- Microtubule-associated proteins 1A/1B light chain 3A (Autophagy-relatedprotein LC3 A) (Autophagy-related ubiquitin-like modifier LC3 A) (MAP1light chain 3-like protein 1) (MAP1A/MAP1B light chain 3 A)(MAP1A/MAP1B LC3 A) (Microtubule-associated protein 1 light chain 3; LC3A; Microtubule-associated protein 1 light chain 3 alpha
- Gene ID
- 84557
- UniProt ID
- Q9H492
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-010R | Mouse Anti-LC3A Recombinant Antibody (ZG-010R) | WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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