Mouse Anti-LC3A Recombinant Antibody (clone 5G10)

Name: Mouse Anti-LC3A Recombinant Antibody (clone 5G10)
SKU: MOB-0086F
Rating: 5 (1 reviews)

CAT#: MOB-0086F

This product is a mouse antibody that recognizes LC3A.

Tested Data Gene Expression

Figure 1 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in IF

Immunofluorescence analysis of Hela cells. 1. CREB-1 polyclonal antibody (red) is diluted 1:200 (overnight at 4°C). The LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 594 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor 488 is diluted 1:1000 (room temperature, 50 minutes).

Figure 2 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in WB

From 1) Hela Cell Lysate, 2) 3T3 CellLysate, 3) Western blot analysis of lysates of rat brain cells, (green) primary antibody diluted 1:1000, overnight at 4°C, Dylight 800 secondary antibody (at 1: Diluted at 10000 for 1 hour at 37°C. (red) Actin β polyclonal antibody was diluted at 1:5000 as a loading control, overnight at 4°C, and Dylight 680 secondary antibody (diluted at 1:10000, at 37°C for 1 hour.

IHC

Figure 3 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in IHC

Immunohistochemical analysis of paraffin-embedded human uterine tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.

IHC

Figure 4 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in IHC

Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.

Figure 5 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in IF

Immunofluorescence analysis of human lung cancer tissue. 1. LC3A mouse monoclonal antibody (5G10) (red) is diluted 1:200 (4°C, overnight). 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Picture B: DAPI. Picture C: The merger of A+B.

Figure 6 Mouse Anti-LC3A Recombinant Antibody (clone 5G10) (MOB-0086F) in WB

Western blot analysis was performed on 1) Hela cell lysate, 2) 3T3 cell lysate, and 3) rat brain tissue lysate using LC3A Mouse mAb diluted 1:1000.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42824/1453_D6_2_selected.jpg

Normal Tissue Figure 2 IHC staining of human caudate

Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52474/ihc_selected.jpg

Normal Tissue Figure 3 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52484/ihc_selected.jpg

Normal Tissue Figure 4 IHC staining of human appendix

Low expression. RNA expression: 13.7 nTPM

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37174/87851_A_1_2_val_selected.jpg

Normal Tissue Figure 5 Colon

Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37174/87851_A_8_3.jpg

Normal Tissue Figure 6 Liver

Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7649/146650_A_7_4.jpg

Normal Tissue Figure 7 Kidney

Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37174/87851_A_8_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37174/87851_A_5_6.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52484/128993_A_8_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (HHSteC, HSkMC, SK-BR-3)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101460-MAP1LC3A

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Specifications

Host Species
Mouse

Type
Mouse IgG

Specificity
Human LC3A

Species Reactivity
Human, Rat, Mouse

Clone
5G10

Applications
WB, IF, IHC-P

Conjugate
Unconjugated

Product Property

Format
Liquid

Storage
Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Western Blot, Immunofluorescence, Immunohistologyp-Paraffin Sections.

Target

Alternative Names
Microtubule-associated proteins 1A/1B light chain 3A (Autophagy-relatedprotein LC3 A) (Autophagy-related ubiquitin-like modifier LC3 A) (MAP1light chain 3-like protein 1) (MAP1A/MAP1B light chain 3 A)(MAP1A/MAP1B LC3 A) (Microtubule-associated protein 1 light chain 3; LC3A; Microtubule-associated protein 1 light chain 3 alpha

Gene ID
84557

UniProt ID
Q9H492

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-0086F.pdf

MSDS

MSDS - MOB-0086F.pdf

COA

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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