Mouse Anti-Lck Monoclonal Antibody (clone 8E5F9)
CAT#: VS7-0425-WR280
This product is a mouse IgG2a antibody that binds to human Lck. The antibody 8E5F9 can be used for WB assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/if_selected.jpg
Normal Tissue
Figure 2 High expression in lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/11345_A_8_8.jpg
Normal Tissue
Figure 3 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/11345_A_4_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182866-LCK
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Specifications
- Immunogen
- Recombinant human Lck fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human Lck
- Species Reactivity
- Human
- Clone
- 8E5F9
- Molecule Class
- Non-Receptor Tyrosine Kinase
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency 22 (IMD22)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LSK; YT16; IMD22; p56lck; pp58lck
- Gene ID
- 3932
- UniProt ID
- P06239
- Long Name
- LCK Proto-Oncogene, Src Family Tyrosine Kinase
- Cellular Localization
- Cell membrane; Cytoplasm; Membrane
- Post Translation Modifications
- Isopeptide bond; Lipoprotein; Myristate; Palmitate; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001036236.1
- Function
- Kinase; Transferase; Tyrosine-protein kinase
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Related Signaling Pathways
TCR Signaling Pathway
Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0525-XY3959 | Anti-LCK Immunohistochemistry Kit | IHC |
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