Mouse Anti-Lck Monoclonal Antibody (clone 8E5F9) (CAT#: VS7-0425-WR280)
This product is a mouse IgG2a antibody that binds to human Lck. The antibody 8E5F9 can be used for WB assay.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/if_selected.jpg
Figure 2 High expression in lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/11345_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/11345_A_8_8.jpg
Figure 3 Tonsil
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/11345_A_4_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/11345_A_4_8.jpg
Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182866-LCK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182866-LCK
Specifications
- Immunogen
- Recombinant human Lck fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human Lck
- Species Reactivity
- Human
- Clone
- 8E5F9
- Molecule Class
- Non-Receptor Tyrosine Kinase
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency 22 (IMD22)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LSK; YT16; IMD22; p56lck; pp58lck
- Gene ID
- 3932
- UniProt ID
- P06239
- Long Name
- LCK Proto-Oncogene, Src Family Tyrosine Kinase
- Cellular Localization
- Cell membrane; Cytoplasm; Membrane
- Post Translation Modifications
- Isopeptide bond; Lipoprotein; Myristate; Palmitate; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001036236.1
- Function
- Kinase; Transferase; Tyrosine-protein kinase
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
TCR Signaling Pathway
Primary Immunodeficiency
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1482z | Mouse Anti-LCK Recombinant Antibody (clone 25C4) | WB, FC, ICC, IHC, IP | Mouse IgG1 |
| MOB-1072CT | Recombinant Mouse anti-Human LCK Monoclonal antibody (9F6G0) | ELISA, WB | |
| ZG-011R | Mouse Anti-LCK Recombinant Antibody (ZG-011R) | WB, ELISA | Mouse IgG |
| VS3-FY843 | Recombinant Rabbit Anti-LCK Antibody (clone R01-8A5) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY2938 | Mouse Anti-LCK Recombinant Antibody (VS3-FY2938) | WB, IHC, ICC, FC | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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