Mouse Anti-LGALS1 Recombinant Antibody (clone 1H11C5) (CAT#: VS7-0425-WR284)
This product is a mouse IgG1 antibody that binds to human LGALS1. The antibody 1H11C5 recognizes specific amino acid domain of human LGALS1 (AA: 1-135). It can be used for IHC and FC assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/687/171_F9_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/687/171_F9_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1130/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1130/if_selected.jpg
Figure 3 IHC staining of human smooth muscle
(Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/646/1191_B_6_7_selected.jpg
(Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/646/1191_B_6_7_selected.jpg
Figure 4 IHC staining of human pancreas
(Low expression. RNA expression: 155.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_2_3_val_selected.jpg
(Low expression. RNA expression: 155.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_2_3_val_selected.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_B_8_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_B_8_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/646/1191_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/646/1191_A_9_3.jpg
Figure 7 Liver
(Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_8_4.jpg
(Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_8_4.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_7_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_7_5.jpg
Figure 9 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_4_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_4_6.jpg
Figure 10 Endometrium
(Cells in endometrial stroma
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_B_5_3.jpg
(Cells in endometrial stroma
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_B_5_3.jpg
Figure 11 Lymph node
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_8_8.jpg
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_8_8.jpg
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100097-LGALS1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100097-LGALS1
Specifications
- Immunogen
- Recombinant human LGALS1 (AA: 1-135) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human LGALS1
- Species Reactivity
- Human
- Clone
- 1H11C5
- Molecule Class
- Galectin
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LGALS1; lectin, galactoside-binding, soluble, 1; GBP; GAL1; galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-gal
- Gene ID
- 3956
- UniProt ID
- P09382
- Long Name
- Galectin 1
- Cellular Localization
- Cytoplasm; Extracellular matrix; Secreted
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_002296.1
- Function
- Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69 (PubMed:24752896).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-622 | Recombinant Anti-human LGALS1 Antibody | WB, Dot, FuncS | IgG |
| MHH-622 | Recombinant Human Anti-human LGALS1 Antibody | ELISA, WB, IF, FuncS | IgG |
| HPAB-0140-FY | Mouse Anti-LGALS1 Recombinant Antibody (HPAB-0140-FY) | Inhib, WB, Neut, IHC, ChIP | Mouse IgG2b, λ |
| FAMAB-1326CQ | Mouse Anti-LGALS1 Recombinant Antibody (clone SAIC-25B-112) | Immuno-MRM, ELISA | Mouse IgG1 |
| VS-0723-WK174 | Human Anti-LGALS1 Recombinant Antibody (VS-0723-WK174) | FC | Chimeric (rabbit/human) IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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