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    Mouse Anti-LGALS1 Recombinant Antibody (clone 1H11C5) (CAT#: VS7-0425-WR284)

    This product is a mouse IgG1 antibody that binds to human LGALS1. The antibody 1H11C5 recognizes specific amino acid domain of human LGALS1 (AA: 1-135). It can be used for IHC and FC assays.

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    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
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    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/687/171_F9_1_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1130/if_selected.jpg
    Figure 3 IHC staining of human smooth muscle
    Figure 3 IHC staining of human smooth muscle
    (Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/646/1191_B_6_7_selected.jpg
    Normal Tissue
    Figure 4 IHC staining of human pancreas
    Figure 4 IHC staining of human pancreas
    (Low expression. RNA expression: 155.5 nTPM)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_2_3_val_selected.jpg
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_B_8_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/646/1191_A_9_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Hepatocytes
    Staining: Not detected
    Intensity: Weak
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_8_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_7_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_4_6.jpg
    Figure 10 Endometrium
    Figure 10 Endometrium
    (Cells in endometrial stroma
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_B_5_3.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2157/5993_A_8_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100097-LGALS1

    Specifications

    • Immunogen
    • Recombinant human LGALS1 (AA: 1-135) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human LGALS1
    • Species Reactivity
    • Human
    • Clone
    • 1H11C5
    • Molecule Class
    • Galectin
    • Applications
    • IHC, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Flow Cytometry: 1:200 - 1:400
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • LGALS1; lectin, galactoside-binding, soluble, 1; GBP; GAL1; galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-gal
    • Long Name
    • Galectin 1
    • Cellular Localization
    • Cytoplasm; Extracellular matrix; Secreted
    • Post Translation Modifications
    • Acetylation; Phosphoprotein
    • Function
    • Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69 (PubMed:24752896).

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "LGALS1"

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    CAT Product Name Application Type
    MOB-622 Recombinant Anti-human LGALS1 Antibody WB, Dot, FuncS IgG
    MHH-622 Recombinant Human Anti-human LGALS1 Antibody ELISA, WB, IF, FuncS IgG
    HPAB-0140-FY Mouse Anti-LGALS1 Recombinant Antibody (HPAB-0140-FY) Inhib, WB, Neut, IHC, ChIP Mouse IgG2b, λ
    FAMAB-1326CQ Mouse Anti-LGALS1 Recombinant Antibody (clone SAIC-25B-112) Immuno-MRM, ELISA Mouse IgG1
    VS-0723-WK174 Human Anti-LGALS1 Recombinant Antibody (VS-0723-WK174) FC Chimeric (rabbit/human) IgG1

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