Human Anti-LGALS2 Recombinant Antibody (clone FF06) (CAT#: VS-0524-YC27)
The antibody was able to bind the recombinant human galectin-2 protein in ELISA experiments. And a stronger intensity signal in the colonic crypts region can be detected in the sections stained with FF06 by immunofluorescence assay.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/1030_D12_2_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/1030_D12_2_selected.jpg
Figure 2 IHC staining of human gallbladder
(High expression. RNA expression: 335.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_4_4_selected.jpg
(High expression. RNA expression: 335.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_4_4_selected.jpg
Figure 3 IHC staining of human skeletal muscle
(Low expression. RNA expression: 1.9 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10742_B_3_6_val_selected.jpg
(Low expression. RNA expression: 1.9 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10742_B_3_6_val_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25198/55091_B_8_5.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25198/55091_B_8_5.jpg
Figure 5 Colon
(Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_9_3.jpg
(Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_9_3.jpg
Figure 6 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_8_4.jpg
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_8_4.jpg
Figure 7 Kidney
(Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity:>75%
Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_8_5.jpg
(Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity:>75%
Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_8_5.jpg
Figure 8 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_9_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3536/10541_A_9_8.jpg
Figure 9 RNA cell line category: Group enriched (CACO-2, Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100079-LGALS2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100079-LGALS2
Specifications
- Host Species
- Human
- Derivation
- Antibody phage display
- Type
- Human IgG
- Specificity
- Human galectin-2
- Species Reactivity
- Human
- Clone
- FF06
- Applications
- ELISA, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS, pH 7.4
- Preservative
- No preservative
- Storage
- Centrifuge briefly prior to opening vial. Store at 4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- HL14
- Gene ID
- 3957
- UniProt ID
- Q6PIT8
- Long Name
- Galectin-2
- Cellular Localization
- Cell membrane; Single-pass type I membrane protein
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3358z | Mouse Anti-LGALS2 Recombinant Antibody (clone 40B1) | WB, IP, IF, ELISA | Mouse IgG2b, κ |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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