CytoStream™ Rabbit Anti-LGALS3 Recombinant Antibody (VS13-YC654) (CAT#: VS13-YC654)
The CytoStream™ Rabbit Anti-LGALS3 Recombinant Antibody is specific for human and mouse LGALS3. LGALS3, part of the galectin family, binds multivalent saccharides and plays roles in apoptosis, immunity, and cell regulation, showing antimicrobial activity with multiple splicing variants. The anti-LGALS3 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76528/2057_C12_5_selected.jpg
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76528/2057_C12_5_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5191/639_D6_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5191/639_D6_1_selected.jpg
Figure 3 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5191/ihc_selected.jpg
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5191/ihc_selected.jpg
Figure 4 IHC staining of human cerebral cortex
(Low expression. RNA expression: 148.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_B_9_5_val_selected.jpg
(Low expression. RNA expression: 148.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_B_9_5_val_selected.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_9_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_9_4.jpg
Figure 6 Kidney
(Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_8_5.jpg
(Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_8_5.jpg
Figure 7 Testis
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_6_6.jpg
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_6_6.jpg
Figure 8 Skin
(Eccrine glands
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Lymphocytes
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_B_7_1.jpg
(Eccrine glands
Staining: High
Intensity: Strong
Quantity:>75%
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Lymphocytes
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_B_7_1.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_7_8.jpg
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3162/8903_A_7_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, CAPAN-2, OE19, SK-MEL-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131981-LGALS3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131981-LGALS3
Specifications
- Immunogen
- Recombinant human Galectin 3, aa 1-49.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse LGALS3
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, FC, IHC-P
- Conjugate
- NONE
- Related Disease
- Follicular Adenoma and Papillary Carcinoma
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Immunohistochemistry-paraffin (IHC-P): 1/200-1/1000
Target
- Alternative Names
- Galectin 3; Advanced Glycation End-Product Receptor 3; Lectin, Galactoside-Binding, Soluble, 3; Carbohydrate-Binding Protein 35; Galactose-Specific Lectin 3; Laminin-Binding Protein; IgE-Binding Protein; 35 KDa Lectin; Lectin L-29; GALBP; MAC2
- Gene ID
- 3958
- UniProt ID
- P17931
- Long Name
- Galectin 3
- Cellular Localization
- Secreted; Nucleus; Cytoplasm; Spliceosome
- Protein Refseq
- NP_002297.2
- Function
- Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1583z | Mouse Anti-LGALS3 Recombinant Antibody (clone 32D7) | WB, ELISA, FC, IHC, FuncS | Mouse IgG1 |
| MRO-0621-CN | Recombinant Rabbit Anti-LGALS3 Monoclonal Antibody (JF39-10) | WB, IF, FC | Rabbit IgG |
| MOB-244YJL | Rabbit Anti-LGALS3 Recombinant Antibody (clone CBL228) | WB, ELISA, FC, ICC, IF, IP | Rabbit IgG |
| MOB-245YJL | Mouse Anti-LGALS3 Recombinant Antibody (clone CBL229) | WB, ELISA, IP | Mouse IgG1 |
| ZG-0176U | Mouse Anti-LGALS3 Recombinant Antibody (clone 3F2E2) | ELISA, WB, IHC | Mouse IgG2b |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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