Rabbit Anti-LGALS4 Recombinant Antibody (clone R02-2B5) (CAT#: VS3-XY3416)
It is a recombinant rabbit antibody targeting the LGALS4 (Galectin 4), which may be involved in the assembly of adherens junctions. It specifically reacts with human and rat LGALS4. It has been determined using Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of Western Blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31185/1394_F2_6_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31185/1394_F2_6_selected.jpg
Figure 2 IHC staining of human colon
(High expression. RNA expression: 1307.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31185/130377_A_8_3_val_selected.jpg
(High expression. RNA expression: 1307.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31185/130377_A_8_3_val_selected.jpg
Figure 3 IHC staining of human cerebral cortex
(Low expression. RNA expression: 0.6 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31185/130377_B_9_5_val_selected.jpg
(Low expression. RNA expression: 0.6 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31185/130377_B_9_5_val_selected.jpg
Figure 4 rectum
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31184/130350_A_6_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31184/130350_A_6_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31184/130350_A_9_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31184/130350_A_9_4.jpg
Figure 6 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31186/155316_A_8_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31186/155316_A_8_5.jpg
Figure 7 Testis
(Leydig cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25653/59323_A_5_6.jpg
(Leydig cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25653/59323_A_5_6.jpg
Figure 8 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171747-LGALS4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171747-LGALS4
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat LGALS4
- Species Reactivity
- Human, Rat
- Clone
- R02-2B5
- Applications
- WB, IHC-P
- Related Disease
- Greig Cephalopolysyndactyly Syndrome; Herpes Simplex
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- GAL4; L36LBP
- Gene ID
- 3960
- UniProt ID
- P56470
- Long Name
- Galectin 4
- Cellular Localization
- Plasma membrane, nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P56470)
Modification sites at neXtProt (NX_P56470)
- Protein Refseq
- XP_054176991.1; XP_011525275.1
- Function
- Galectin that binds lactose and a related range of sugars.
May be involved in the assembly of adherens junctions.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "LGALS4"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2434MZ | Recombinant Mouse Anti-Human LGALS4 Antibody (clone NN0400-2E23) | WB | Mouse antibody |
| VS-0524-YC25 | Human Anti-LGALS4 Recombinant Antibody (clone FF04) | ELISA, IF | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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