Rabbit Anti-LGR5 Recombinant Antibody (clone 4H8)
CAT#: ZG-0792J
This product is a rabbit antibody that recognizes LGR5.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-LGR5 Antibody (ZG-0792J) in WB
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: LGR5 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 100, 98, 93 kDa
Observed band size: 100 kDa
FC
Figure 2 Rabbit Anti-LGR5 Antibody (ZG-0792J) in FC
Overlay histogram showing HepG2 cells stained with this product (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
❮
❯
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/1404_H2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/103_B2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/102_B2_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139292-LGR5
❮
❯
Specifications
- Immunogen
- A synthesized peptide derived from human LGR5/GPR49
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LGR5
- Species Reactivity
- Human, Rat
- Clone
- 4H8
- Applications
- ELISA, WB, FC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry.
WB:1:500-1:5000, FC:1:20-1:200
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0792J. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "LGR5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1650-VHH | Recombinant Anti-Human LGR5 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2770z | Mouse Anti-LGR5 Recombinant Antibody (clone 44A6) | FC, ICC, FuncS | Mouse IgG2a |
| HPAB-0105-CN | Human Anti-LGR5 Recombinant Antibody (HPAB-0105-CN) | WB, ELISA, FC, IHC | Chimeric (mouse/human) IgG1, κ |
| MRO-1905-CN | Rabbit Anti-LGR5 Polyclonal Antibody (MRO-1905-CN) | WB, IHC | Rabbit IgG |
| VS3-CJ177 | Rabbit Anti-LGR5 Recombinant Antibody (VS3-CJ177) | WB, IP, FC | Rabbit IgG |
| VS7-0425-WR285 | Mouse Anti-LGR5 Recombinant Antibody (clone 2B5B9) | FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-490CL | Human Anti-LGR5 Recombinant Antibody (TAB-490CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-199CT | Human Anti-LGR5 Recombinant Antibody (TAB-199CT) | ELISA, FC, RIA, IP, WB | Chimeric antibody (mouse/human) |
| TAB-199CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-199CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| TAB-199CT-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (TAB-199CT-F(E)) | ELISA, FC, RIA, IP, WB | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-196CT-S(P) | Rabbit Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-196CT-S(P)) | IHC, FC, ELISA, WB | Rabbit scFv |
| TAB-197CT-S(P) | Rabbit Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-197CT-S(P)) | IHC, FC, ELISA, WB | Rabbit scFv |
| TAB-198CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-198CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| TAB-200CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-200CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| TAB-201CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-201CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2048 | Hi-Affi™ Recombinant Rabbit Anti-LGR5 Monoclonal Antibody (DS2048AB) | WB, IP, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0101-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| HPAB-0102-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0102-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| HPAB-0103-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0103-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| HPAB-0104-CN-S(P) | Human Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0104-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| HPAB-0106-CN-S(P) | Human Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0106-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0101-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0102-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0102-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0103-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0103-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0104-CN-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0104-CN-F(E)) | WB, ELISA, FC, IHC | Human Fab |
| HPAB-0105-CN-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0105-CN-F(E)) | WB, ELISA, FC, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY32 | Mouse Anti-LGR5 Agonistic Antibody (VS-0724-XY32) | FC, Agonistic assays, CyTOF®, ICC | Mouse IgG2a |
| VS-0724-XY33 | Rat Anti-Lgr5 Agonistic Antibody (VS-0724-XY33) | FC, Agonistic assays, CyTOF®, ICC | Rat IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY173 | AbPlus™ Anti-LGR5 Magnetic Beads (VS-0424-XY173) | IP, Protein Purification | |
| VS-0724-YC625 | AbPlus™ Anti-LGR5 Magnetic Beads (VS-0724-YC625) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC71 | Rabbit Anti-LGR5 Antibody (VS-0924-YC71) - Cancer Stem Cell Marker | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC634 | Recombinant Anti-LGR5 Vesicular Antibody, EV Displayed (VS-0425-YC634) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4011 | Anti-LGR5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4012 | Anti-Mouse LGR5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC118 | Recombinant Anti-LGR5 (AA 22-322 x AA 323-558) Biparatopic Antibody, Tandem scFv | ELISA, FC, IHC, WB | Tandem scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, Neut, FuncS
-YJ5.png)
Application: WB, ELISA, FC, IHC, IP
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR
Application: WB, ELISA, FC, IHC, IF, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
