Mouse Anti-LIMS1 Monoclonal Antibody (clone 5G7)
CAT#: VS7-0425-WR615
This product is a mouse IgG1 antibody that binds to human LIMS1. The antibody 5G7 can be used for ICC and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58455/976_B1_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58455/135870_B_4_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58455/135870_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_4_6.jpg
Normal Tissue
Figure 8 Fallopian tube
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_9_7.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169756-LIMS1
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Specifications
- Immunogen
- Recombinant human LIMS1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human LIMS1
- Species Reactivity
- Human
- Clone
- 5G7
- Molecule Class
- LIM-Domain-Containing Adaptor Proteins
- Applications
- ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LIMS1; LIM And Senescent Cell Antigen-Like-Containing Domain Protein 1; Particularly Interesting New Cys-His Protein 1; PINCH1; LIM And Senescent Cell Antigen-Like Domains 1; Renal Carcinoma Antigen NY-REN-48
- Gene ID
- 3987
- UniProt ID
- P48059
- Long Name
- LIM zinc finger domain containing 1
- Cellular Localization
- Cell junction; Cell membrane; Membrane
- Post Translation Modifications
- Acetylation
- Protein Refseq
- NP_001180411.1
- Function
- Actin-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 5G7"
- CAT
- Product Name
See other products for "LIMS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3396z | Mouse Anti-LIMS1 Recombinant Antibody (clone 30G8) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MOB-2478CT | Recombinant Mouse anti-Human LIMS1 Monoclonal antibody (5G7) | WB, IP, ELISA | |
| VS3-QX689 | Mouse Anti-LIMS1 Recombinant Antibody (clone 3C12-F7-A8) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY1012 | Mouse Anti-LIMS1 Recombinant Antibody (clone 5G7B9) | ICC, FC | Mouse IgG1 |
| VS7-0425-WR299 | Mouse Anti-LIMS1 Monoclonal Antibody (VS7-0425-WR299) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2059 | Hi-Affi™ Recombinant Rabbit Anti-LIMS1 Monoclonal Antibody (DS2059AB) | WB, FC | IgG |
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