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    Mouse Anti-LIMS1 Monoclonal Antibody (clone 5G7) (CAT#: VS7-0425-WR615)

    This product is a mouse IgG1 antibody that binds to human LIMS1. The antibody 5G7 can be used for ICC and FC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & focal adhesion sites.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/58455/976_B1_3_selected.jpg
    Figure 2 IHC staining of human adrenal gland
    Figure 2 IHC staining of human adrenal gland
    (Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/58455/135870_B_4_5_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Neuronal cells
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/58455/135870_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_7_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_4_6.jpg
    Figure 8 Fallopian tube
    Figure 8 Fallopian tube
    (Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_9_7.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_8_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Cell line enhanced (HMC-1)
    Figure 10 RNA cell line category: Cell line enhanced (HMC-1)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169756-LIMS1

    Specifications

    • Immunogen
    • Recombinant human LIMS1 fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human LIMS1
    • Species Reactivity
    • Human
    • Clone
    • 5G7
    • Molecule Class
    • LIM-Domain-Containing Adaptor Proteins
    • Applications
    • ICC, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Preservative
    • 0.03% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunocytochemistry: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • LIMS1; LIM And Senescent Cell Antigen-Like-Containing Domain Protein 1; Particularly Interesting New Cys-His Protein 1; PINCH1; LIM And Senescent Cell Antigen-Like Domains 1; Renal Carcinoma Antigen NY-REN-48
    • Long Name
    • LIM zinc finger domain containing 1
    • Cellular Localization
    • Cell junction; Cell membrane; Membrane
    • Post Translation Modifications
    • Acetylation
    • Function
    • Actin-binding

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "Clone 5G7"

    See other products for "LIMS1"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibody Products Rabbit Monoclonal Antibody Products
    CAT Product Name Application Type
    MOB-3396z Mouse Anti-LIMS1 Recombinant Antibody (clone 30G8) WB, ELISA, FC, ICC, IF Mouse IgG1
    MOB-2478CT Recombinant Mouse anti-Human LIMS1 Monoclonal antibody (5G7) WB, IP, ELISA
    VS3-QX689 Mouse Anti-LIMS1 Recombinant Antibody (clone 3C12-F7-A8) WB, IHC, ICC, FC, ELISA Mouse IgG1
    VS3-XY1012 Mouse Anti-LIMS1 Recombinant Antibody (clone 5G7B9) ICC, FC Mouse IgG1
    VS7-0425-WR299 Mouse Anti-LIMS1 Monoclonal Antibody (VS7-0425-WR299) WB, IF, ICC, FC, ELISA Mouse IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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