Mouse Anti-LIMS1 Monoclonal Antibody (VS7-0425-WR299)
CAT#: VS7-0425-WR299
This product is a mouse IgG antibody that binds to human LIMS1. The antibody can be used for WB, IF, ICC, FC, and ELISA assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58455/976_B1_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58455/135870_B_4_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58455/135870_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_4_6.jpg
Normal Tissue
Figure 8 Fallopian tube
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_9_7.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61230/135814_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169756-LIMS1
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Specifications
- Immunogen
- Recombinant human LIMS1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human LIMS1
- Species Reactivity
- Human
- Molecule Class
- Adaptor Protein
- Applications
- WB, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LIMS1; LIM And Senescent Cell Antigen-Like-Containing Domain Protein 1; Particularly Interesting New Cys-His Protein 1; PINCH1; LIM And Senescent Cell Antigen-Like Domains 1; Renal Carcinoma Antigen NY-REN-48
- Gene ID
- 3987
- UniProt ID
- P48059
- Long Name
- LIM zinc finger domain containing 1
- Cellular Localization
- Cell junction; Cell membrane; Membrane
- Post Translation Modifications
- Acetylation
- Protein Refseq
- NP_001180411.1
- Function
- Actin-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "LIMS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3396z | Mouse Anti-LIMS1 Recombinant Antibody (clone 30G8) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MOB-2478CT | Recombinant Mouse anti-Human LIMS1 Monoclonal antibody (5G7) | WB, IP, ELISA | |
| VS3-QX689 | Mouse Anti-LIMS1 Recombinant Antibody (clone 3C12-F7-A8) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY1012 | Mouse Anti-LIMS1 Recombinant Antibody (clone 5G7B9) | ICC, FC | Mouse IgG1 |
| VS7-0425-WR615 | Mouse Anti-LIMS1 Monoclonal Antibody (clone 5G7) | ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2059 | Hi-Affi™ Recombinant Rabbit Anti-LIMS1 Monoclonal Antibody (DS2059AB) | WB, FC | IgG |
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