Mouse Anti-LMNA Recombinant Antibody (VS3-CJ299)
CAT#: VS3-CJ299
This product is a mouse antibody that recognizes LMNA.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6523/1905_E12_1_selected.jpg
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human, Mouse and Rat LMNA
- Species Reactivity
- Human, Mouse, Rat
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A/G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:4000).
Target
- Alternative Names
- FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
- Gene ID
- 4000
- UniProt ID
- P02545
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Intermediate filament, Nucleus
- Post Translation Modifications
- Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Phosphorylation status of S-22 determines its localization between double-strand break (DSB) sites and the nuclear matrix (PubMed:31548606).
Proteolytic cleavage of the C-terminal of 18 residues of prelamin-A/C results in the production of lamin-A/C. The prelamin-A/C maturation pathway includes farnesylation of CAAX motif, ZMPSTE24/FACE1 mediated cleavage of the last three amino acids, methylation of the C-terminal cysteine and endoproteolytic removal of the last 15 C-terminal amino acids. Proteolytic cleavage requires prior farnesylation and methylation, and absence of these blocks cleavage.
Sumoylation is necessary for the localization to the nuclear envelope.
Farnesylation of prelamin-A/C facilitates nuclear envelope targeting.
- Protein Refseq
- NP_001244303.1; NP_001269553.1; NP_001269554.1
- Function
- Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606).
Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:22431096, PubMed:10814726, PubMed:11799477, PubMed:18551513).
Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980).
Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585).
Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920).
Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "LMNA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1798z | Mouse Anti-LMNA Recombinant Antibody (clone 12B7) | ICC, IF, IHC | Mouse IgG3 |
| MOB-1769MZ | Recombinant Mouse Anti-Human LMNA Antibody (clone KPM3) | FC, ICC, IF, IHC-Fr, IHC-P, IP, WB | Mouse antibody |
| ZG-0958F | Mouse Anti-LMNA Recombinant Antibody (clone 131C3) | FC, ICC, IHC-Fr | Mouse IgG1 |
| ZG-0960F | Mouse Anti-LMNA Recombinant Antibody (clone 133A2) | FC, ICC, IHC-Fr | Mouse IgG3 |
| VS1-YJ190 | Mouse Anti-LMNA Recombinant Antibody (clone 13A4D4) | WB, ICC, IF, IP | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0327MZ | Chicken Anti-LMNA Polyclonal IgY | WB | Chicken antibody |
| BRD-1186MZ | Chicken Anti-Lamin A/C Polyclonal IgY | N/A | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2067 | Hi-Affi™ Recombinant Rabbit Anti-LMNA Monoclonal Antibody (DS2067AB) | FC, ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1922 | PE-A*02:01/Human LMNA (RLAVYIDRV) MHC Tetramer | FCM | |
| MHC-CN0129 | APC-B*58:01/Human Lamin (KAGQVVTIW) MHC Tetramer | FCM | |
| MHC-CN0130 | PE-B*58:01/Human Lamin (KAGQVVTIW) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY175 | AbPlus™ Anti-LMNA Magnetic Beads (13A4D4) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC665 | CytoStream™ Rabbit Anti-LMNA Recombinant Antibody (VS13-YC665) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4051 | Anti-LMNA Immunohistochemistry Kit | IHC | |
| VS-0525-XY4052 | Anti-Mouse LMNA Immunohistochemistry Kit | IHC |
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