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    Rabbit Anti-LMNB1 Recombinant Antibody (VS13-YC666) (CAT#: VS13-YC666)

    The Rabbit Anti-LMNB1 Recombinant Antibody is specific for human, mouse and rat LMNB1. LMNB1 encodes B-type lamin proteins, part of the nuclear lamina, with duplication leading to leukodystrophy. The anti-LMNB1 antibody can be used for Western blot (1/500-1/1000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/100-1/500).

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    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line MCF7
    Figure 1 IF staining of human cell line MCF7
    (Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/if_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/950_C1_1_selected.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/79757/1894_G3_4_selected.jpg
    Figure 4 IHC staining of human colon
    Figure 4 IHC staining of human colon
    (Immunohistochemical staining of human colon shows nuclear membrane positivity in glandular and stroma cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/ihc_selected.jpg
    Figure 5 IHC staining of human stomach
    Figure 5 IHC staining of human stomach
    (Immunohistochemical staining of human stomach shows strong nuclear membranous positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/155655_A_3_1_selected.jpg
    Figure 6 IHC staining of human esophagus
    Figure 6 IHC staining of human esophagus
    (Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/79954/171056_B_6_1_selected.jpg
    Normal Tissue
    Figure 7 Cerebral cortex
    Figure 7 Cerebral cortex
    (Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_B_8_5.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cholangiocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_7_4.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Leydig cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_5_6.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_8_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Low cell line specificity
    Figure 11 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113368-LMNB1

    Specifications

    • Immunogen
    • Recombinant human Lamin B1, aa 511-560.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat LMNB1
    • Species Reactivity
    • Human, Mouse, Rat
    • Applications
    • WB, ICC, IF, IHC-P, IP
    • Conjugate
    • NONE

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A purified
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
    • Preservative
    • 0.05% Sodium azide
    • Storage
    • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Shipping with ice pack.

    Applications

    • Application Notes
    • Western blot (WB): 1/500-1/1000
      Immunocytochemistry (ICC): 1/50-1/200
      Immunofluorescence (IF): 1/50-1/200
      Immunohistochemistry-paraffin (IHC-P): 1/100-1/500

    Target

    • Alternative Names
    • LMN2, LMNB
    • Long Name
    • Lamin B1
    • Sequence Similarities
    • Belongs to the intermediate filament family.
    • Cellular Localization
    • Nucleus inner membrane; Intermediate filament
    • Function
    • Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "LMNB1"

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    CAT Product Name Application Type
    MOB-1818z Mouse Anti-LMNB1 Recombinant Antibody (clone 19G6) ELISA, IHC, WB Mouse IgG2a, κ
    MOB-2469CT Recombinant Mouse anti-Chicken LMNB1 Monoclonal antibody (T70) WB
    MRO-0913-CN Recombinant Rabbit Anti-LMNB1 Monoclonal Antibody (CBACN-342) WB, IF, IHC, IP Rabbit IgG
    MRO-0914-CN Recombinant Rabbit Anti-LMNB1 Monoclonal Antibody (JM099-03) WB, IHC, FC Rabbit IgG
    VS3-QX694 Mouse Anti-LMNB1 Recombinant Antibody (clone 5G8-D3-H7) WB Mouse IgG2b

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