Mouse Anti-LPA Monoclonal Antibody (clone 8F6A9)
CAT#: VS7-0425-WR305
This product is a mouse IgG1 antibody that binds to human LPA. The antibody 8F6A9 recognizes specific amino acid domain of LPA (4330-4521). It can be used for IHC assay.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human liver
Immunohistochemical staining of human liver shows moderate cytoplasmic and membranous positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_A_8_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows distinc extra cellular and some, weaker membranous and cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16072/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_A_4_6.jpg
Normal Tissue
Figure 6 Skin
Epidermal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_B_6_8.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16072/36402_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198670-LPA
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Specifications
- Immunogen
- Recombinant LPA (4330-4521) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human LPA
- Species Reactivity
- Human
- Clone
- 8F6A9
- Molecule Class
- Glycerophospholipids
- Applications
- IHC
- Conjugate
- Unconjugated
- Related Disease
- Atherosclerosis
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LPA; Lysophosphatidic acid
- Gene ID
- 4018
- UniProt ID
- P08519
- Long Name
- Lipoprotein(a)
- Cellular Localization
- extracellular region; extracellular space
- Post Translation Modifications
- Autocatalytic cleavage; Disulfide bond; Glycoprotein
- Protein Refseq
- NP_005568.2
- Function
- Hydrolase; Protease; Serine protease
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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