Mouse Anti-LTF Recombinant Antibody (VS3-CJ316) (CAT#: VS3-CJ316)

• Immunogen
• Synthetic peptide

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human LTF

• Species Reactivity
• Human

• Applications
• IHC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50% Glycerol, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Immunohistochemistry (1:50-1:300).

• Alternative Names
• LF; Lactotransferrin; Lactoferrin; Talalactoferrin; HLF2; GIG12; HEL110

• Gene ID
• 4057

• UniProt ID
• P02788

• Sequence Similarities
• Belongs to the transferrin family.

• Cellular Localization
• Cytoplasm, Nucleus, Secreted

• Post Translation Modifications
• Isoform DeltaLf:
  Phosphorylation at Ser-10 activates the transcriptional activity (PubMed:20404350).
  Phosphorylation at Ser-10 also promotes proteasomal degradation (PubMed:20404350).
  Alternatively can undergo O-GlcNAcylation at Ser-10 (PubMed:20404350).
  Isoform DeltaLf:
  O-GlcNAcylation at Ser-10 inhibits DNA binding and negatively regulates the transcriptional activity (PubMed:20404350).
  Alternatively can undergo phosphorylation at Ser-10 (PubMed:20404350).
  Poly-N-acetyllactosaminic carbohydrate moiety seems to be needed for TLR4 activation.

• Protein Refseq
• NP_001186078.1; NP_001308050.1; NP_001308051.1

• Function
• Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.
  Lactotransferrin: Major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions (PubMed:14573629, PubMed:1599934, PubMed:6802759, PubMed:3169987, PubMed:11179314, PubMed:12693969).
  Has antimicrobial activity, which depends on the extracellular cation concentration (PubMed:6802759).
  Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane (PubMed:14573629, PubMed:1599934, PubMed:6802759, PubMed:3169987, PubMed:11179314, PubMed:12693969).
  Can also prevent bacterial biofilm development in P.aeruginosa infection (PubMed:12037568).
  Has weak antifungal activity against C.albicans (PubMed:11083624).
  Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth (PubMed:15166119).
  Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection (PubMed:17079302).
  Can inhibit papillomavirus infections (PubMed:17481742).
  Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling (PubMed:20345905).
  Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells (PubMed:19033648).
  Stimulates VEGFA-mediated endothelial cell migration and proliferation (PubMed:16842782).
  Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs) (PubMed:9359845).
  Also binds specifically to pneumococcal surface protein A (PspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA (PubMed:9359845).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS