Rabbit Anti-MAP1LC3B Recombinant Antibody (clone R06-4K9)
CAT#: VS3-XY3425
This is a recombinant rabbit antibody that specifically recognizes human MAP1LC3B (Microtubule associated protein 1 light chain 3 beta), which promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. It also has cross-reactivity against mouse or rat MAP1LC3B. It has been reported for use in Western Blot, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53767/965_A12_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42824/1453_D6_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_B_7_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140941-MAP1LC3B
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MAP1LC3B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-4K9
- Applications
- WB, ICC, IF, IP
- Related Disease
- Pancreatic Ductal Adenocarcinoma; Liver Carcinoma In Situ
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
- Gene ID
- 81631
- UniProt ID
- Q9GZQ8
- Long Name
- Microtubule associated protein 1 light chain 3 beta
- Cellular Localization
- Cytoplasm, cytoskeleton, Cytoplasmic vesicle
- Post Translation Modifications
- Phosphorylation by PKA inhibits conjugation of phosphatidylethanolamine (PE) (PubMed:22948227).
Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity (PubMed:22948227).
(Q9GZQ8-MLP3B_HUMAN)
Modification sites at PhosphoSitePlus (Q9GZQ8).
Modification sites at neXtProt (NX_Q9GZQ8).
- Protein Refseq
- NP_073729.1
- Function
- Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, 23209295, 28017329).
Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, 28017329).
In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP1LC3B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4640 | Hi-Affi™ Recombinant Rabbit Anti-MAP1LC3B Monoclonal Antibody (TH153DS) | WB, IF, ICC, IHC-P, FC, ChIP | IgG |
| MOR-0046-FY | Rabbit Anti-MAP1LC3B Recombinant Antibody (clone AFY0017) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2008-CN | Rabbit Anti-MAP1LC3B Polyclonal Antibody (MRO-2008-CN) | WB, IF, IHC | Rabbit IgG |
| MOB-0087F | Mouse Anti-MAP1LC3B Recombinant Antibody (clone 9H5) | IHC-P, IF | Mouse IgG |
| VS3-WK1139 | Mouse Anti-MAP1LC3B Recombinant Antibody (VS3-WK1139) | WB, IHC, IF | Mouse IgG |
| VS3-WK1141 | Rabbit Anti-MAP1LC3B Recombinant Antibody (VS3-WK1141) | WB, IP | Rabbit IgG |
| VS7-0425-WR330 | Mouse Anti-MAP1LC3B Monoclonal Antibody (clone 5H12) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1051 | AbPlus™ Anti-MAP1LC3B Magnetic Beads (VS-0724-YC1051) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4197 | Anti-MAP1LC3B Immunohistochemistry Kit | IHC | |
| VS-0525-XY4198 | Anti-Mouse MAP1LC3B Immunohistochemistry Kit | IHC | |
| VS-0525-XY4199 | Anti-Rat MAP1LC3B Immunohistochemistry Kit | IHC |
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