Rabbit Anti-MAP1LC3B Recombinant Antibody (clone R06-4K9) (CAT#: VS3-XY3425)
This is a recombinant rabbit antibody that specifically recognizes human MAP1LC3B (Microtubule associated protein 1 light chain 3 beta), which promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. It also has cross-reactivity against mouse or rat MAP1LC3B. It has been reported for use in Western Blot, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53767/965_A12_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53767/965_A12_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42824/1453_D6_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42824/1453_D6_2_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_B_7_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_B_7_5.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_7_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37216/152162_A_5_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140941-MAP1LC3B
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140941-MAP1LC3B
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MAP1LC3B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-4K9
- Applications
- WB, ICC, IF, IP
- Related Disease
- Pancreatic Ductal Adenocarcinoma; Liver Carcinoma In Situ
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
- Gene ID
- 81631
- UniProt ID
- Q9GZQ8
- Long Name
- Microtubule associated protein 1 light chain 3 beta
- Cellular Localization
- Cytoplasm, cytoskeleton, Cytoplasmic vesicle
- Post Translation Modifications
- Phosphorylation by PKA inhibits conjugation of phosphatidylethanolamine (PE) (PubMed:22948227).
Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity (PubMed:22948227).
(Q9GZQ8-MLP3B_HUMAN)
Modification sites at PhosphoSitePlus (Q9GZQ8).
Modification sites at neXtProt (NX_Q9GZQ8).
- Protein Refseq
- NP_073729.1
- Function
- Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, 23209295, 28017329).
Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, 28017329).
In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4640 | Hi-Affi™ Recombinant Rabbit Anti-MAP1LC3B Monoclonal Antibody (TH153DS) | WB, IF, ICC, IHC-P, FC, ChIP | IgG |
| MOR-0046-FY | Rabbit Anti-MAP1LC3B Recombinant Antibody (clone AFY0017) | ICC, IF, IHC-P, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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