Rabbit Anti-MAP2K3 Recombinant Antibody (clone R06-1F1) (CAT#: VS3-FY1765)
Clone R06-1F1 is a rabbit recombinant monoclonal antibody that confidently identify human MAP2K3. It cross-reacts with mouse, rat. The antibody is recommended for detection of MAP2K3 protein by WB, ICC/IF, IP. This MEK3 antibody was verified by western blot analysis of MEK3 in C6, 3T3, Hela lysates.
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WB
Figure 1 Anti-MAP2K3 Antibody (VS3-FY1765) in WB.
The MEK3 expression in C6, 3T3, Hela lysates were detected using the anti-MEK3 by western blot assay.
Specifications
- Immunogen
- Recombinant human MEK3 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MAP2K3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-1F1
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 38-40 kDa.
- Related Disease
- Pulmonary Systemic Sclerosis and Inhalation Anthrax
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase 3; MAP Kinase Kinase 3; MAPK/ERK Kinase 3; Stress-Activated Protein Kinase Kinase 2; SAPK Kinase 2; EC 2.7.12.2; MAPKK 3; SAPKK-2; PRKMK3
- Gene ID
- 5606
- UniProt ID
- P46734
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Cellular Localization
- cytoplasm, cytosol, membrane, nucleoplasm
- Post Translation Modifications
- Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases positively regulates the kinase activity (PubMed:8622669).
Phosphorylated by TAOK2.
- Protein Refseq
- XP_011522260.1; XP_011522261.1; XP_016880346.2; XP_016880348.1; XP_047292364.1
- Function
- Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1458MZ | Recombinant Mouse Anti-Human MAP2K3 Antibody (clone 2E20) | IHC-P, WB, sELISA | Mouse antibody |
| MRO-0981-CN | Recombinant Rabbit Anti-MAP2K3 Monoclonal Antibody (CBACN-360) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-0982-CN | Recombinant Rabbit Anti-MAP2K3 Monoclonal Antibody (CBACN-361) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS-0923-FY116 | Recombinant Mouse Anti-MAP2K3 Antibody (VS-0923-FY116) | ELISA, WB | Mouse IgG2a |
| VS7-0425-WR334 | Mouse Anti-MAP2K3 Recombinant Antibody (clone 2E12D11) | IHC, ICC, FC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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