Mouse Anti-MAP3K11 Recombinant Antibody (clone 3D11) (CAT#: VS-0625-FY40)
The Mouse IgG2a kappa monoclonal antibody, clone 3D11, is designed for detecting human MAP3K11. Its immunogen is a recombinant human MAP3K11 protein fragment, with an epitope mapping to amino acids 741-847. With an approximate antigen size of 47.7 kDa, this antibody is applicable in Western Blot (1:500), ELISA, FuncS, and IHC, offering versatile detection capabilities.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & centriolar satellites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/970_H1_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & centriolar satellites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/970_H1_3_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_B_9_5.jpg
(Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_B_9_5.jpg
Figure 3 Lung
(Alveolar cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Macrophages Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_2_4.jpg
(Alveolar cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Macrophages Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_2_4.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_7_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_7_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10165/65747_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173327-MAP3K11
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173327-MAP3K11
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a, kappa
- Species Reactivity
- Human
- Clone
- 3D11
- Applications
- Western Blot, ELISA, Functional Assay, Immunohistochemistry
- Related Disease
- Dementia and Gastric Cancer
Product Property
- Purification
- Purified with Protein A or G affinity chromatography
- Purity
- >90% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.4
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500
Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase Kinase 11; Src-Homology 3 Domain-Containing Proline-Rich Kinase; Mixed Lineage Kinase 3; EC 2.7.11.25; MLK3; PTK1; SPRK
- Gene ID
- 4296
- UniProt ID
- Q16584
- Long Name
- Mitogen-Activated Protein Kinase Kinase Kinase 11
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
- Cellular Localization
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
- Post Translation Modifications
- Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
Thr-277 is likely to be the main autophosphorylation site.
Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
- Protein Refseq
- XP_047282918.1; XP_054224804.1; NP_002410.1
- Function
- Activates the JUN N-terminal pathway.
Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Endometrial Cancer
Throid Cancer
MAPK Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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