Mouse Anti-MAPK1 Recombinant Antibody (clone 1H4) (CAT#: VS3-WK1581)
This antibody is a mouse recombinant antibody against human MAPK1, which has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The clone 1H4 can be used in WB analysis at a dilution of 1/1000-1/2000 and in IHC analysis at a dilution of 1/100-200.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79930/1999_H7_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79930/1999_H7_3_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal and glial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3995/12643_B_9_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal and glial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3995/12643_B_9_5_selected.jpg
Figure 3 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_7_6_selected.jpg
(Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_7_6_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_8_5.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_8_5.jpg
Figure 5 Colon
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_3.jpg
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_3.jpg
Figure 6 Liver
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_7_4.jpg
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_7_4.jpg
Figure 7 Pancreas
(Exocrine glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Pancreatic endocrine cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_2_3.jpg
(Exocrine glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Pancreatic endocrine cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_2_3.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_5.jpg
(Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_5.jpg
Figure 9 Testis
(Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_6_6.jpg
(Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_6_6.jpg
Figure 10 Lymph node
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_8.jpg
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_8.jpg
Figure 11 RNA cell line category: Cell line enhanced (K-562)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100030-MAPK1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100030-MAPK1
Figure 11 RNA cell line category: Cell line enhanced (K-562)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100030-MAPK1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100030-MAPK1
Specifications
- Immunogen
- Synthetic peptide of ERK1/2 at AA range of 140-220
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MAPK1
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 1H4
- Applications
- WB, IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/1000-1/2000
IHC: 1/100-1/200
Target
- Alternative Names
- ERK; p38; p40; p41; ERK2; ERT1; NS13; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK
- Gene ID
- 5594
- UniProt ID
- P27361
- Long Name
- Mitogen-activated protein kinase 1
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Cell junction, Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylated upon KIT and FLT3 signaling (By similarity).
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
- Protein Refseq
- NP_001035145.1
- Function
- Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
FcεR1 Signaling Pathway
TCR Signaling Pathway
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1334z | Mouse Anti-MAPK1 Recombinant Antibody (clone 22C9) | ELISA, IHC, WB | Mouse IgG2b |
| MRO-1846-CN | Rabbit Anti-MAPK1 Polyclonal Antibody (MRO-1846-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0315J | Mouse Anti-MAPK1 Recombinant Antibody (ZG-0315J) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0455U | Rabbit Anti-MAPK1 Recombinant Antibody (clone 10C7) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-XY1038 | Mouse Anti-MAPK1 Recombinant Antibody (clone 2G1) | WB, IP, IF | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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