Mouse Anti-MAPK7 Recombinant Antibody (clone 6D10)
CAT#: VS3-WK1614
This antibody is a mouse recombinant antibody against human MAPK7, which is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. The clone 6D10 can be used in WB analysis at a dilution of 1/1000-1/2000 and IHC analysis at a dilution of 1/50-1/200.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31031/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/if_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_9_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_5.jpg
Normal Tissue
Figure 7 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_3_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, SuSa)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166484-MAPK7
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Specifications
- Immunogen
- Synthesized peptide derived from human ERK5
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MAPK7
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 6D10
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC-P: 1/50-1/200
WB: 1/1000-1/2000
Target
- Alternative Names
- BMK1; ERK4; ERK5; PRKM7
- Gene ID
- 5598
- UniProt ID
- Q13164
- Long Name
- Mitogen-activated protein kinase 7
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity).
Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
- Protein Refseq
- NP_002740.2
- Function
- Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via interaction with STUB1/CHIP and promotion of STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
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Related Signaling Pathways
FcεR1 Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
Isotype Control
- CAT
- Product Name
See other products for "MAPK7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2798z | Mouse Anti-MAPK7 Recombinant Antibody (clone 14C6) | WB, FC, ICC, IF | Mouse IgG1 |
| MRO-0549-CN | Recombinant Rabbit Anti-MAPK7 Monoclonal Antibody (CBACN-219) | WB, IF, IP, FC | Rabbit IgG |
| VS3-XY1042 | Mouse Anti-MAPK7 Recombinant Antibody (clone 8H1D5) | IHC | Mouse IgG1 |
| VS3-WK919 | Rabbit Anti-MAPK7 Recombinant Antibody (VS3-WK919) | WB, ICC, IF, IP, FC | Rabbit IgG |
| VS3-FY888 | Recombinant Mouse Anti-MAPK7 Antibody (clone 4D6) | IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0344MZ | Chicken Anti-MAPK7 (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0345MZ | Chicken Anti-MAPK7 (ab2) Polyclonal IgY | WB | Chicken antibody |
| BRD-0929MZ | Chicken Anti-ERK5 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2158 | Hi-Affi™ Recombinant Rabbit Anti-MAPK7 Monoclonal Antibody (DS2158AB) | WB, ICC, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1301 | Anti-MAPK7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4243 | Anti-Human MAPK7 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC697 | CytoStream™ Rabbit Anti-MAPK7 Recombinant Antibody (VS13-YC697) | WB, ICC, IF, IP, FC | Rabbit IgG |
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