CytoStream™ Rabbit Anti-MAPK7 Recombinant Antibody (VS13-YC697) (CAT#: VS13-YC697)
The CytoStream™ Rabbit Anti-MAPK7 Recombinant Antibody is specific for human and mouse MAPK7. MAPK7 encodes a member of the MAP kinase family, activated by MAP2K5/MEK5. It's involved in signaling processes downstream of various receptors, translocating to the nucleus in response to signals to regulate gene expression by phosphorylating transcription factors. There are four alternatively spliced transcript variants encoding two isoforms. The anti-MAPK7 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31031/if_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31031/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/if_selected.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_8.jpg
Figure 4 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_9_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_9_5.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_4.jpg
Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_5.jpg
Figure 7 Skeletal muscle
(Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_3_6.jpg
(Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_3_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, SuSa)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166484-MAPK7
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166484-MAPK7
Specifications
- Immunogen
- Recombinant human ERK5, aa 767-816.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MAPK7
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 7; Extracellular-Signal-Regulated Kinase 5; Big MAP Kinase 1; MAP Kinase 7; EC 2.7.11.24; BMK1 Kinase; MAPK 7; PRKM7;
- Gene ID
- 5598
- UniProt ID
- Q13164
- Long Name
- Mitogen-Activated Protein Kinase 7
- Cellular Localization
- Nucleus; Cytoplasm
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
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TCR Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2798z | Mouse Anti-MAPK7 Recombinant Antibody (clone 14C6) | WB, FC, ICC, IF | Mouse IgG1 |
| MRO-0549-CN | Recombinant Rabbit Anti-MAPK7 Monoclonal Antibody (CBACN-219) | WB, IF, IP, FC | Rabbit IgG |
| VS3-FY888 | Recombinant Mouse Anti-MAPK7 Antibody (clone 4D6) | IHC | Mouse IgG |
| VS3-FY889 | Recombinant Mouse Anti-MAPK7 Antibody (clone 4G5) | IHC-p, WB | Mouse IgG |
| VS3-WK1614 | Mouse Anti-MAPK7 Recombinant Antibody (clone 6D10) | IHC-P, WB | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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