Rabbit Anti-MAPKAPK3 Recombinant Antibody (clone R08-4B6)
CAT#: VS3-FY2036
The R08-4B6 recombinant monoclonal antibody has been genetically engineered to specifically target human MAPKAPK3. It is suitable for use in a variety of assays, including WB, IP. This MAPKAP kinase 3 antibody was used in western blot analysis of MK3 in A549, HL-60 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-MAPKAPK3 Antibody (VS3-FY2036) in WB.
The MK3 expression in A549, HL-60 lysates were detected using the anti-MAPKAP Kinase 3 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58275/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000114738-MAPKAPK3
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Specifications
- Immunogen
- Synthetic peptide of human MK-3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAPKAPK3
- Species Reactivity
- Human
- Clone
- R08-4B6
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 43 kDa, while the observed molecular weight stands at 43 kDa.
- Related Disease
- Macular Dystrophy, Patterned, 3 and Bacteremia 2
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Mitogen-Activated Protein Kinase-Activated Protein Kinase 3; MAPK-Activated Protein Kinase 3; Chromosome 3p Kinase; MAPKAP Kinase 3; EC 2.7.11.1; MAPKAP-K3; MAPKAPK-3
- Gene ID
- 7867
- UniProt ID
- Q16644
- Sequence Similarities
- Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1.
Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313 ().
(Q16644-MAPK3_HUMAN)
Modification sites at PhosphoSitePlus (Q16644)
Modification sites at neXtProt (NX_Q16644)
Glycosylation from GlyGen (Q16644) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047304839.1; XP_047304840.1; XP_047304841.1; XP_047304842.1; XP_047304843.1
- Function
- MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAPKAPK3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1202CT | Recombinant Mouse anti-Human MAPKAPK3 Monoclonal antibody (EML2251) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0347MZ | Chicken Anti-MAPKAPK3 (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0348MZ | Chicken Anti-MAPKAPK3 (ab2) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2162 | Hi-Affi™ Recombinant Rabbit Anti-MAPKAPK3 Monoclonal Antibody (DS2162AB) | WB, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4251 | Anti-MAPKAPK3 Immunohistochemistry Kit | IHC |
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