Rabbit Anti-MAPT (pS324) Recombinant Antibody (clone R01-9K4)
CAT#: VS3-WK2173
This product is a rabbit recombinant antibody that is specific to phosphorylated MAPT. The target of this antibody is phospho-MAPT (Ser324). The clone R01-9K4 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200 and IP analysis at a dilution of 1/20. The molecular weight of MAPT (pSer324) observed in WB experiments using this antibody is 79 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48895/1070_E6_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunohistochemical staining of human appendix shows strong positivity in peripheral ganglia and nerve fibers.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69524/156431_A_3_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining:
Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Proximal tubules (cell body)
Staining:
Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HDLM-2, SCLC-21H, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186868-MAPT
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser324 of human, mouse and rat MAPT
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-9K4
- Applications
- WB, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- Microtubule Associated Protein Tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; Protein Phosphatase 1, Regulatory Subunit 103; Neurofibrillary Tangle Protein; Paired Helical Filament-Tau; PHF-Tau; MAPTL; MTBT1; TAU
- Gene ID
- 4137
- UniProt ID
- P10636
- Long Name
- Microtubule Associated Protein Tau
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-674 | Chicken Anti-MAPT Recombinant Antibody (clone pT231/pS235_1); scFv Fragment | WB, ELISA, FACS, Block | Chicken scFv |
| PSBL-705 | Mouse Anti-MAPT Recombinant Antibody (clone DC8E8); scFv Fragment | WB, IF, FuncS | Mouse scFv |
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| HPAB-0568-WJ-S(P) | Mouse Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0568-WJ-S(P)) | ELISA | Mouse scFv |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-4714 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH228DS) | WB, IF, ICC, IHC-P, FC | IgG |
| MOR-4715 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH229DS) | WB, IF, ICC, IHC-P | IgG |
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4255 | Anti-MAPT Immunohistochemistry Kit | IHC | |
| VS-0525-XY4256 | Anti-Mouse MAPT Immunohistochemistry Kit | IHC | |
| VS-0525-XY4257 | Anti-Rat MAPT Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC120 | Recombinant Anti-MAPT (AA 220-224 x AA 506-511) Biparatopic Antibody, Tandem scFv (Clone 13A6 x Clone 19G10) | ELISA | Tandem scFv |
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