Rabbit Anti-MAPT (pT231) Recombinant Antibody (clone R01-2A9) (CAT#: VS3-WK2129)
This product is a rabbit recombinant antibody that is specific to phosphorylated MAPT. The target of this antibody is phospho-MAPT (Thr231). The clone R01-2A9 can be used in WB analysis at a dilution of 1/500-1/1000, IP analysis at a dilution of 1/20, and IHC staining at a dilution of 1/50-1/100. The molecular weight of MAPT (pThr231) observed in WB experiments using this antibody is 46 kDa.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48895/1070_E6_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48895/1070_E6_2_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunohistochemical staining of human appendix shows strong positivity in peripheral ganglia and nerve fibers.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69524/156431_A_3_2_selected.jpg
(Immunohistochemical staining of human appendix shows strong positivity in peripheral ganglia and nerve fibers.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69524/156431_A_3_2_selected.jpg
Figure 3 Cerebral cortex
(Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_B_8_5.jpg
(Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_B_8_5.jpg
Figure 4 Colon
(Endocrine cells
Staining:
Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_3.jpg
(Endocrine cells
Staining:
Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_3.jpg
Figure 5 Kidney
(Proximal tubules (cell body)
Staining:
Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_5.jpg
(Proximal tubules (cell body)
Staining:
Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_5.jpg
Figure 6 RNA cell line category: Group enriched (HDLM-2, SCLC-21H, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186868-MAPT
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186868-MAPT
Figure 6 RNA cell line category: Group enriched (HDLM-2, SCLC-21H, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186868-MAPT
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186868-MAPT
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr231 of human, mouse and rat MAPT
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-2A9
- Applications
- WB, IHC-P, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
- Alternative Names
- Microtubule Associated Protein Tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; Protein Phosphatase 1, Regulatory Subunit 103; Neurofibrillary Tangle Protein; Paired Helical Filament-Tau; PHF-Tau; MAPTL; MTBT1; TAU
- Gene ID
- 4137
- UniProt ID
- P10636
- Long Name
- Microtubule Associated Protein Tau
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-674 | Chicken Anti-MAPT Recombinant Antibody (clone pT231/pS235_1); Fab Fragment | WB, ELISA, FACS, Block | Chicken Fab |
| PFBL-705 | Mouse Anti-MAPT Recombinant Antibody (clone DC8E8); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBL-706 | Rabbit Anti-MAPT Recombinant Antibody Fab Fragment (PFBL-706) | WB, IF, FuncS | Rabbit Fab |
| HPAB-0042-WJ-F(E) | Human Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-0042-WJ-F(E)) | ELISA, WB | Humanized Fab |
| HPAB-0196-YJ-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-0196-YJ-F(E)) | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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