Rabbit Anti-MARS Recombinant Antibody (clone R02-2B7)
CAT#: VS3-FY1767
This R02-2B7 recombinant monoclonal antibody has been genetically engineered to specifically target human MARS. It is suitable for use in a variety of assays, including WB, ICC/IF. This MetRS antibody has been rigorously validated and is widely used in western blot analysis of methionyl tRNA synthetase in K562, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/if_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_9_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_9_3.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Strong Quantity: <25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (RH-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166986-MARS
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WB
Figure 1 Anti-MARS Antibody (VS3-FY1767) in WB.
The Methionyl tRNA synthetase expression in K562, Hela lysates were detected using the anti-MetRS by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human methionyl-tRNA synthetase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MARS
- Species Reactivity
- Human
- Clone
- R02-2B7
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 101 kDa, while the observed molecular weight stands at 101 kDa.
- Related Disease
- Interstitial Lung And Liver Disease and Charcot-Marie-Tooth Disease, Axonal, Type 2U
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Methionyl-TRNA Synthetase; EC 6.1.1.10; METRS; Methionine TRNA Ligase 1, Cytoplasmic; Methionine--TRNA Ligase, Cytoplasmic; Cytosolic Methionyl-TRNA Synthetase; CMT2U
- Gene ID
- 4141
- UniProt ID
- P56192
- Sequence Similarities
- Belongs to the class-I aminoacyl-tRNA synthetase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- XP_047284807.1; XP_047284808.1; XP_054228032.1; XP_054228033.1; NP_004981.2
- Function
- Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Plays a role in the synthesis of ribosomal RNA in the nucleolus.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MARS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2170 | Hi-Affi™ Recombinant Rabbit Anti-MARS Monoclonal Antibody (DS2170AB) | WB, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-439C | Human Anti-MARS Recombinant Antibody (ZG-439C) | ELISA, IP | Human IgG1, κ |
| VS3-FY2796 | Rabbit Anti-MARS Recombinant Antibody (VS3-FY2796) | WB, IHC, ICC, IP | Rabbit IgG |
| VS13-YC706 | Rabbit Anti-MARS Recombinant Antibody (VS13-YC706) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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