Rabbit Anti-MARS Recombinant Antibody (VS3-FY2796)
CAT#: VS3-FY2796
This anti-MARS antibody is a rabbit recombinant monoclonal antibody that has been designed to bind the human MARS protein. It cross-reacts with rat species. The monoclonal antibody can be used in the area of Interstitial Lung And Liver Disease and Charcot-Marie-Tooth Disease, Axonal, Type 2U. The antibody can be utilized for detection of target protein by Immunoprecipitation (1/20), Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100) and Immunocytochemistry (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/if_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_9_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_9_3.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Strong Quantity: <25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17097/38706_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (RH-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166986-MARS
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat MARS
- Species Reactivity
- Human, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Interstitial Lung And Liver Disease and Charcot-Marie-Tooth Disease, Axonal, Type 2U
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Methionyl-TRNA Synthetase; EC 6.1.1.10; METRS; Methionine TRNA Ligase 1, Cytoplasmic; Methionine--TRNA Ligase, Cytoplasmic; Cytosolic Methionyl-TRNA Synthetase; CMT2U
- Gene ID
- 4141
- UniProt ID
- P56192
- Sequence Similarities
- Belongs to the class-I aminoacyl-tRNA synthetase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- XP_047284807.1; XP_047284808.1; XP_054228032.1; XP_054228033.1; NP_004981.2
- Function
- Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:11714285).
Plays a role in the synthesis of ribosomal RNA in the nucleolus (PubMed:10791971).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MARS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2170 | Hi-Affi™ Recombinant Rabbit Anti-MARS Monoclonal Antibody (DS2170AB) | WB, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-439C | Human Anti-MARS Recombinant Antibody (ZG-439C) | ELISA, IP | Human IgG1, κ |
| VS3-FY1767 | Rabbit Anti-MARS Recombinant Antibody (clone R02-2B7) | WB, IF | Rabbit IgG |
| VS13-YC706 | Rabbit Anti-MARS Recombinant Antibody (VS13-YC706) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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