Mouse Anti-MASTL Recombinant Antibody (VS3-FY2797) (CAT#: VS3-FY2797)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human MASTL

• Species Reactivity
• Human

• Applications
• Western Blot

• Conjugate
• Unconjugated, FITC

• MW
• The molecular weight of the target protein is approximately 97.3 kDa.

• Related Disease
• Autosomal Thrombocytopenia With Normal Platelets and Thrombocytopenia 2

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/2000

• Alternative Names
• Microtubule Associated Serine/Threonine Kinase Like; Greatwall Kinase Homolog; MAST-L; THC2; GWL; GW; Microtubule-Associated Serine/Threonine-Protein Kinase-Like

• Gene ID
• 84930

• UniProt ID
• Q96GX5

• Sequence Similarities
• Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

• Cellular Localization
• Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  Nucleus
  Cleavage furrow

• Post Translation Modifications
• Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity).
  Maximum phosphorylation occurs in prometaphase.
  (Q96GX5-GWL_HUMAN)
  Ubiquitination at Lys39, Lys179, Lys199, and Lys269 (NX_Q96GX5 [NX_Q96GX5-1])
  Modification sites at PhosphoSitePlus (Q96GX5)
  Glycosylation from GlyGen (Q96GX5) 2 sites, 1 O-linked glycan (2 sites)

• Protein Refseq
• XP_005252689.1; XP_006717582.1; XP_006717583.1; XP_011518053.1

• Function
• Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively.
  ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A.
  Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high.
  Following DNA damage, it is also involved in checkpoint recovery by being inhibited.
  Phosphorylates histone protein in vitro; however such activity is unsure in vivo.
  May be involved in megakaryocyte differentiation.

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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