Mouse Anti-MCM2 Recombinant Antibody (clone ABT-MCM2) (CAT#: VS3-CJ992)

• Immunogen
• Synthesized peptide derived from human MCM2

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human MCM2

• Species Reactivity
• Human

• Clone
• ABT-MCM2

• Applications
• WB, IHC-P

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:20-1:100).

• Alternative Names
• BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN

• Gene ID
• 4171

• UniProt ID
• P49736

• Sequence Similarities
• Belongs to the MCM family.

• Cellular Localization
• Chromosome, Nucleus

• Post Translation Modifications
• Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.

• Protein Refseq
• NP_004517.2

• Function
• Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
  The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
  Required for the entry in S phase and for cell division (PubMed:8175912).
  Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed:26196677).

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS