Mouse Anti-MCM2 Recombinant Antibody (clone PT2030)
CAT#: VS3-CJ993
This product is a mouse antibody that recognizes human MCM2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31450/1923_C3_26_cr5cd2b031ba569_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31495/721_D2_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31496/727_C2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_4_6.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_B_6_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073111-MCM2
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Specifications
- Immunogen
- Synthesized peptide derived from human MCM2
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human MCM2
- Species Reactivity
- Human
- Clone
- PT2030
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
- Gene ID
- 4171
- UniProt ID
- P49736
- Sequence Similarities
- Belongs to the MCM family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
- Protein Refseq
- NP_004517.2
- Function
- Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Required for the entry in S phase and for cell division (PubMed:8175912).
Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed:26196677).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MCM2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0715MZ | Chicken Anti-MCM2 Polyclonal IgY | ICC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2190 | Hi-Affi™ Recombinant Rabbit Anti-MCM2 Monoclonal Antibody (DS2190AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0101F | Mouse Anti-MCM2 Recombinant Antibody (MOB-0101F) | IHC-P, IF | Mouse IgG |
| VS3-QX762 | Mouse Anti-MCM2 Recombinant Antibody (clone 2B5-H4-A9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY907 | Recombinant Rabbit Anti-MCM2 (phospho Ser108) Antibody (clone R01-7G9) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY908 | Recombinant Rabbit Anti-MCM2 (phospho Ser40) Antibody (clone R03-1B3) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY909 | Recombinant Rabbit Anti-MCM2 (phospho Ser41) Antibody (clone R07-4C3) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY318 | Mouse Anti-NHP MCM2 Recombinant Antibody (clone 2B3) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1319 | Anti-MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4304 | Anti-Mouse MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4303 | Anti-Human MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4305 | Anti-Rat MCM2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC711 | CytoStream™ Rabbit Anti-MCM2 Recombinant Antibody (VS13-YC711) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| VS13-YC712 | CytoStream™ Mouse Anti-MCM2 Recombinant Antibody (VS13-YC712) | WB, IHC, IF, IP, FC | Mouse IgG1 |
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