CytoStream™ Rabbit Anti-MCM2 Recombinant Antibody (VS13-YC711)
CAT#: VS13-YC711
The CytoStream™ Rabbit Anti-MCM2 Recombinant Antibody is specific for human MCM2. MCM2, part of mini-chromosome maintenance proteins, is crucial for the initiation of genome replication, forming a complex with MCM4, 6, and 7 for helicase activity regulation. Phosphorylated by CDC2/CDC7, it has spliced variants of unknown full-length nature. The anti-MCM2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/500-1/1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31450/1923_C3_26_cr5cd2b031ba569_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31495/721_D2_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31496/727_C2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_4_6.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_B_6_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073111-MCM2
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Specifications
- Immunogen
- Recombinant MCM2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MCM2
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/2000
Immunohistochemistry-paraffin (IHC-P): 1/800-1/2000
Immunocytochemistry (ICC): 1/50
Flow cytometry (FC): 1/500-1/1000
Target
- Alternative Names
- Minichromosome Maintenance Complex Component 2; Minichromosome Maintenance Protein 2 Homolog; Nuclear Protein BM28; Mitotin; CCNL1; CDCL1; BM28; MCM2 Minichromosome Maintenance Deficient 2, Mitotin (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin); Minichromosome Maintenance Deficient 2 (Mitotin);
- Gene ID
- 4171
- UniProt ID
- P49736
- Long Name
- Minichromosome Maintenance Complex Component 2
- Sequence Similarities
- Belongs to the MCM family.
- Cellular Localization
- Nucleus; Chromosome
- Protein Refseq
- NP_004517.2
- Function
- Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Required for the entry in S phase and for cell division (PubMed:8175912).
Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed:26196677).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MCM2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0715MZ | Chicken Anti-MCM2 Polyclonal IgY | ICC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2190 | Hi-Affi™ Recombinant Rabbit Anti-MCM2 Monoclonal Antibody (DS2190AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK2074 | Rabbit Anti-MCM2 (pS27) Recombinant Antibody (clone R88-9Q-1) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-WK2075 | Rabbit Anti-MCM2 (pS108) Recombinant Antibody (clone R47-7H-3) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR368 | Mouse Anti-MCM2 Monoclonal Antibody (clone 1E7) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR369 | Mouse Anti-MCM2 Monoclonal Antibody (VS7-0425-WR369) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR370 | Mouse Anti-MCM2 Monoclonal Antibody (VS7-0425-WR370) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY318 | Mouse Anti-NHP MCM2 Recombinant Antibody (clone 2B3) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1319 | Anti-MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4304 | Anti-Mouse MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4303 | Anti-Human MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4305 | Anti-Rat MCM2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC712 | CytoStream™ Mouse Anti-MCM2 Recombinant Antibody (VS13-YC712) | WB, IHC, IF, IP, FC | Mouse IgG1 |
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