CytoStream™ Rabbit Anti-MCM2 Recombinant Antibody (VS13-YC711) (CAT#: VS13-YC711)

• Immunogen
• Recombinant MCM2.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human MCM2

• Species Reactivity
• Human

• Applications
• WB, IHC-P, ICC, IF, FC

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/2000
  Immunohistochemistry-paraffin (IHC-P): 1/800-1/2000
  Immunocytochemistry (ICC): 1/50
  Flow cytometry (FC): 1/500-1/1000

• Alternative Names
• Minichromosome Maintenance Complex Component 2; Minichromosome Maintenance Protein 2 Homolog; Nuclear Protein BM28; Mitotin; CCNL1; CDCL1; BM28; MCM2 Minichromosome Maintenance Deficient 2, Mitotin (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin); Minichromosome Maintenance Deficient 2 (Mitotin);

• Gene ID
• 4171

• UniProt ID
• P49736

• Long Name
• Minichromosome Maintenance Complex Component 2

• Sequence Similarities
• Belongs to the MCM family.

• Cellular Localization
• Nucleus; Chromosome

• Protein Refseq
• NP_004517.2

• Function
• Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
  The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
  Required for the entry in S phase and for cell division (PubMed:8175912).
  Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed:26196677).

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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