Rabbit Anti-MCM3 Recombinant Antibody (clone R04-6B9)
CAT#: VS3-FY1596
Clone R04-6B9 is a rabbit recombinant monoclonal antibody that confidently identify human MCM3. It cross-reacts with rat. The antibody is recommended for detection of MCM3 protein by WB, IHC-F, IHC-P, ICC/IF. The MCM3 antibody is recommended for detection of target protein by western blot analysis of MCM3 in Jurkat, C6, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/13_D12_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/15794_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112118-MCM3
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WB
Figure 1 Anti-MCM3 Antibody (VS3-FY1596) in WB.
The MCM3 expression in Jurkat, C6, Hela lysates were detected using the anti-MCM3 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human MCM3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat MCM3
- Species Reactivity
- Human, Rat
- Clone
- R04-6B9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 91 kDa, while the observed molecular weight stands at 100 kDa.
- Related Disease
- Anaplastic Astrocytoma and Cervical Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Minichromosome Maintenance Complex Component 3; DNA Polymerase Alpha Holoenzyme-Associated Protein P1; RLF Subunit Beta; P1-MCM3; P102; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 3; MCM3 Minichromosome Maintenance Deficient 3; Replication Licensing Factor, Beta Subunit; Minichromosome Maintenance Deficient 3
- Gene ID
- 4172
- UniProt ID
- P25205
- Sequence Similarities
- Belongs to the MCM family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Acetylated by MCM3AP., O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
- Protein Refseq
- NP_001257401.1; NP_002379.3
- Function
- Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Required for the entry in S phase and for cell division (Probable).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| MOB-2628z | Mouse Anti-MCM3 Recombinant Antibody (clone 35B1) | WB, ICC, IF, IHC, IP | Mouse IgG1, κ |
| MOB-0323MZ | Recombinant Mouse Anti-Human MCM3 Antibody (clone A102) | IHC | Mouse antibody |
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| VS3-FY911 | Recombinant Mouse Anti-MCM3 Antibody (clone 1D4A3) | ELISA, WB, IHC, ICC, FC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2191 | Hi-Affi™ Recombinant Rabbit Anti-MCM3 Monoclonal Antibody (DS2191AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1320 | Anti-MCM3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4308 | Anti-Mouse MCM3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4307 | Anti-Human MCM3 Immunohistochemistry Kit | IHC |
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