Rabbit Anti-MCM3 Recombinant Antibody (VS13-YC713)
CAT#: VS13-YC713
The Rabbit Anti-MCM3 Recombinant Antibody is specific for human MCM3. MCM3 is part of the mini-chromosome maintenance protein complex, interacting with MCM2-7 for replication initiation. It interacts with MCM5/CDC46, and its acetylation inhibits replication initiation and cell cycle progression, with two isoforms found. The anti-MCM3 antibody can be used for Immunohistochemistry (1/100-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/13_D12_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/15794_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112118-MCM3
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Specifications
- Immunogen
- Recombinant human MCM3.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MCM3
- Species Reactivity
- Human
- Applications
- IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.1) containing 50% Glycerol and 0.5% BSA.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/100-1/200
Target
- Alternative Names
- Minichromosome Maintenance Complex Component 3; DNA Polymerase Alpha Holoenzyme-Associated Protein P1; RLF Subunit Beta; P1-MCM3; P102; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 3; MCM3 Minichromosome Maintenance Deficient 3; Replication Licensing Factor, Beta Subunit; Minichromosome Maintenance Deficient 3;
- Gene ID
- 4172
- UniProt ID
- P25205
- Long Name
- Minichromosome Maintenance Complex Component 3
- Sequence Similarities
- Belongs to the MCM family.
- Cellular Localization
- Chromosome; Nucleus
- Protein Refseq
- NP_001257401.1; NP_002379.3
- Function
- Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Required for the entry in S phase and for cell division (Probable).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MCM3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2628z | Mouse Anti-MCM3 Recombinant Antibody (clone 35B1) | WB, ICC, IF, IHC, IP | Mouse IgG1, κ |
| MOB-0323MZ | Recombinant Mouse Anti-Human MCM3 Antibody (clone A102) | IHC | Mouse antibody |
| MRO-0972-CN | Recombinant Rabbit Anti-MCM3 Monoclonal Antibody (JB99-35) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-FY911 | Recombinant Mouse Anti-MCM3 Antibody (clone 1D4A3) | ELISA, WB, IHC, ICC, FC | Mouse IgG1, κ |
| VS3-FY1596 | Rabbit Anti-MCM3 Recombinant Antibody (clone R04-6B9) | WB, IHC-Fr, IHC-P, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2191 | Hi-Affi™ Recombinant Rabbit Anti-MCM3 Monoclonal Antibody (DS2191AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1320 | Anti-MCM3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4308 | Anti-Mouse MCM3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4307 | Anti-Human MCM3 Immunohistochemistry Kit | IHC |
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