Mouse Anti-MCM3 Recombinant Antibody (VS3-CJ819) (CAT#: VS3-CJ819)
This product is a mouse antibody that recognizes human, mouse, and rat MCM3.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/13_D12_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/13_D12_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/if_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/ihc_selected.jpg
Figure 4 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/ihc_selected.jpg
Figure 5 Colon
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_8_3.jpg
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_8_3.jpg
Figure 6 Testis
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_6_6.jpg
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/15794_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/15794_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112118-MCM3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112118-MCM3
Specifications
- Immunogen
- Recombinant protein of human MCM3
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat MCM3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:4000), Flow Cytometry (1:25).
Target
- Alternative Names
- HCC5; P1.h; RLFB; P1-MCM3
- Gene ID
- 4172
- UniProt ID
- P25205
- Sequence Similarities
- Belongs to the MCM family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Acetylated by MCM3AP.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
- Protein Refseq
- NP_001257401.1; NP_002379.3
- Function
- Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Required for the entry in S phase and for cell division (Probable).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0323MZ | Recombinant Mouse Anti-Human MCM3 Antibody (clone A102) | IHC | Mouse antibody |
| MRO-0972-CN | Recombinant Rabbit Anti-MCM3 Monoclonal Antibody (JB99-35) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK339 | Rabbit Anti-MCM3 Recombinant Antibody (clone JB99-35) | WB, ELISA | Rabbit IgG |
| VS3-FY911 | Recombinant Mouse Anti-MCM3 Antibody (clone 1D4A3) | ELISA, WB, IHC, ICC, FC | Mouse IgG1, κ |
| VS13-YC713 | Rabbit Anti-MCM3 Recombinant Antibody (VS13-YC713) | IHC-P | Rabbit IgG |
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