Human Anti-MDA-ApoB100 Recombinant Antibody (clone 2D03) (CAT#: HPAB-0259CQ)

Figure 1 Immunohistochemical staining of consecutive sections of aortic plaques from mice injected with control antibody and with 2D03 antibody.

In (A and D), the sections were stained with a secondary anti human IgG to visualize endogenously present antibody. Sections in (B and E) are both stained in vitro with additional 2D03 antibody to visualize the total available 2D03 epitopes. In (C and F), the sections are stained for macrophages (anti-MOMA).

Örbom, A., Jansson, B., Schiopu, A., Evans-Axelsson, S., Nilsson, J., Fredrikson, G. N., & Strand, S. E. (2014). Multi-radionuclide digital autoradiography of the intra-aortic atherosclerotic plaques using a monoclonal antibody targeting oxidized low-density lipoprotein. American journal of nuclear medicine and molecular imaging, 4(2), 172.

Figure 2 Images of the aorta from one animal.

From left to right, the aorta was stained with Oil Red O to identify the lipid deposits followed by the processed Oil Red O image to define plaque and non-plaque areas. Finally, with a 5x5-pixel mean filter applied, digital autoradiography images of the same aorta, separated into contributions from the different radiotracers. Each aorta is individually scaled from zero (white) to max (black) uptake and does not reflect differences in total uptake between different tracers.

Örbom, A., Jansson, B., Schiopu, A., Evans-Axelsson, S., Nilsson, J., Fredrikson, G. N., & Strand, S. E. (2014). Multi-radionuclide digital autoradiography of the intra-aortic atherosclerotic plaques using a monoclonal antibody targeting oxidized low-density lipoprotein. American journal of nuclear medicine and molecular imaging, 4(2), 172.

Figure 3 The plaque-to-aorta radionuclide uptake contrast was highest for the 2D03 antibody in both studies, irrespectively of the tracer or amount of antibody used.

The plaque to aorta radioactivity concentration ratio as determined by dividing the mean image intensity in areas of autoradiography images defined as plaques by that of areas defined as aorta wall for each separated radionuclide image and each animal in both studies (A). And the total specific activity in the mounted aortas as determined by quantified autoradiography images of ¹²⁵I-labelled 2D03 and ¹³¹I-labelled control antibody for study 2 (B).

Örbom, A., Jansson, B., Schiopu, A., Evans-Axelsson, S., Nilsson, J., Fredrikson, G. N., & Strand, S. E. (2014). Multi-radionuclide digital autoradiography of the intra-aortic atherosclerotic plaques using a monoclonal antibody targeting oxidized low-density lipoprotein. American journal of nuclear medicine and molecular imaging, 4(2), 172.

Figure 4 Binding of 2D03 to MDA-modified LDL. The MDA-LDL antigen was coated on test plates and bound antibody was detected with peroxidaselabelled anti-human IgG1.

KD values were estimated after Biacore measurements against MDA-modified apoB-100. The IgG1 FITC-8 antibody was used as negative control.

Ström, Å., Fredrikson, G. N., Schiopu, A., Ljungcrantz, I., Söderberg, I., Jansson, B.,... & Nilsson, J. (2007). Inhibition of injury-induced arterial remodelling and carotid atherosclerosis by recombinant human antibodies against aldehyde-modified apoB-100. Atherosclerosis, 190(2), 298-305.

Figure 5 Luminescence ELISA illustrating the binding of 2D03 antibody and control (FITC-8) to MDA-modified human LDL (MDA-LDL), MDAmodified human apoB-100 (MDA-apoB), unmodified LDL (Na-LDL) and unmodified apoB-100 (Na-apoB).

Ström, Å., Fredrikson, G. N., Schiopu, A., Ljungcrantz, I., Söderberg, I., Jansson, B.,... & Nilsson, J. (2007). Inhibition of injury-induced arterial remodelling and carotid atherosclerosis by recombinant human antibodies against aldehyde-modified apoB-100. Atherosclerosis, 190(2), 298-305.