Human Anti-MDA-ApoB100 Recombinant Antibody (clone IEI-E3) (CAT#: HPAB-0256CQ)

Figure 1 Antibody-Induced Regression of Plaque Area in the Descending Aorta Assessed by Oil Red O Staining.

The values are expressed as the percentages of total plaque area per total area of the descending aorta. p values were calculated versus fluorescein isothiocyanate-8 (FITC-8). **p < 0.01 and ***p < 0.001 versus FITC-8.

Schiopu, A., Frendéus, B., Jansson, B., Söderberg, I., Ljungcrantz, I., Araya, Z.,... & Fredrikson, G. N. (2007). Recombinant antibodies to an oxidized low-density lipoprotein epitope induce rapid regression of atherosclerosis in Apobec-1−/−/low-density lipoprotein receptor−/− mice. Journal of the American College of Cardiology, 50(24), 2313-2318.

Figure 2 Recombinant Human Antibodies to MDA–apoB-100 Peptides Upregulate ABCA-1 Expression In Vivo and In Vitro.

Flow cytometry histogram showing ABCA-1 expression of untreated (gray shaded peak), FITC-8–treated (black line overlay), and 2D03-treated (red line overlay) cells.

Schiopu, A., Frendéus, B., Jansson, B., Söderberg, I., Ljungcrantz, I., Araya, Z.,... & Fredrikson, G. N. (2007). Recombinant antibodies to an oxidized low-density lipoprotein epitope induce rapid regression of atherosclerosis in Apobec-1−/−/low-density lipoprotein receptor−/− mice. Journal of the American College of Cardiology, 50(24), 2313-2318.

Figure 3 Recombinant Human Antibodies to MDA–apoB-100 Peptides Upregulate ABCA-1 Expression In Vivo and In Vitro.

ABCA-1 staining of aortic arch plaques from FITC-8–, IEI-E3–, and 2D03-treated mice.

Schiopu, A., Frendéus, B., Jansson, B., Söderberg, I., Ljungcrantz, I., Araya, Z.,... & Fredrikson, G. N. (2007). Recombinant antibodies to an oxidized low-density lipoprotein epitope induce rapid regression of atherosclerosis in Apobec-1−/−/low-density lipoprotein receptor−/− mice. Journal of the American College of Cardiology, 50(24), 2313-2318.

Figure 4 Antibodies to MDA–apoB-100 Peptides Block oxLDL-Induced Monocyte MCP-1 Production In Vitro.

MCP-1 levels in conditioned medium from monocytes incubated with titrated concentrations of IEI-E3 (shaded squares), 2D03 (solid squares), or FITC-8 control IgG1 (open squares)in the presence of oxLDL-containing human serum. The graphshows mean ± SD of triplicate values from a representative experiment.

Schiopu, A., Frendéus, B., Jansson, B., Söderberg, I., Ljungcrantz, I., Araya, Z.,... & Fredrikson, G. N. (2007). Recombinant antibodies to an oxidized low-density lipoprotein epitope induce rapid regression of atherosclerosis in Apobec-1−/−/low-density lipoprotein receptor−/− mice. Journal of the American College of Cardiology, 50(24), 2313-2318.

Figure 5 Antibodies to MDA–apoB-100 Peptides Block oxLDL-Induced Monocyte MCP-1 Production In Vitro.

MCP-1messenger ribonucleic acid (mRNA) relative to 18SmRNA levels in human monocytes incubated with 60 μg/ml 2D03, IEI-E3, or FITC-8 in the presence of oxLDL-containing human serum. Graphsshow mean ± SD of triplicate values using cells obtained from 3 different donors (n = 9).

Schiopu, A., Frendéus, B., Jansson, B., Söderberg, I., Ljungcrantz, I., Araya, Z.,... & Fredrikson, G. N. (2007). Recombinant antibodies to an oxidized low-density lipoprotein epitope induce rapid regression of atherosclerosis in Apobec-1−/−/low-density lipoprotein receptor−/− mice. Journal of the American College of Cardiology, 50(24), 2313-2318.

Figure 6 ELISA based analysis of binding of 2D03 and IEI-E3 antibodies to MDA-modified human ApoB-100.

Control corresponds to binding of FITC-8 antibody to the same antigen. Affinities were determined by Biacore analysis.