AbPlus™ Anti-MDH1 Magnetic Beads (VS-0724-YC1098)
CAT#: VS-0724-YC1098
The AbPlus Anti-MDH1 Magnetic Beads (VS-0724-YC1098) is an innovative affinity resin which is bound with anti-MDH1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of MDH1 protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27296/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27296/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach, lower
Immunohistochemical staining of human stomach, Lower shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54276/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach (lower)
Immunohistochemical staining of human stomach
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47333/ihc_selected.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27296/60078_B_6_6.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27296/60078_B_7_5.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27296/60078_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000014641-MDH1
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-MDH1 specific antibody
- Target
- MDH1
- Immunogen
- Recombinant human MDH1 fragment derived from E. coli.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016]
- Alternative Names
- Malate Dehydrogenase 1; Malate Dehydrogenase 1, NAD (Soluble); Cytosolic Malate Dehydrogenase; Diiodophenylpyruvate Reductase; EC 1.1.1.37; MDHA; Epididymis Secretory Protein Li 32; Malate Dehydrogenase, Cytoplasmic;
- Gene ID
- 4190
- UniProt ID
- P40925
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "MDH1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1010CT | Recombinant Mouse anti-Human MDH1 Monoclonal antibody (EML578) | WB | |
| VS-0923-FY109 | Recombinant Mouse Anti-MDH1 Antibody (VS-0923-FY109) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2196 | Hi-Affi™ Recombinant Rabbit Anti-MDH1 Monoclonal Antibody (DS2196AB) | ICC, IF, IP, FC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1096 | AbPlus™ Anti-Mdh1 Magnetic Beads (VS-0724-YC1096) | IP, Protein Purification |
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