Mouse Anti-MDM4 Recombinant Antibody (ZG-0275C)
CAT#: ZG-0275C
This product is a mouse antibody that recognizes MDM4. It can be used in a wide variety of applications, such as: WB, IHC, IF, ELISA.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-MDM4 Antibody (ZG-0275C) in WB
Western Blot analysis using MDMX monoclonal antibody against HeLa (1), A549 (2) and A431 (3) cell lysate.
IHC
Figure 2 Mouse Anti-MDM4 Antibody (ZG-0275C) in IHC
Immunohistochemistry analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDMX monoclonal antibody.
IF
Figure 3 Mouse Anti-MDM4 Antibody (ZG-0275C) in IF
Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using MDMX monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48821/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18919/44759_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18919/44759_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18919/44759_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18919/44759_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18919/44759_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18919/44759_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198625-MDM4
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Specifications
- Immunogen
- Purified recombinant fragment of human MDMX expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MDM4
- Species Reactivity
- Human
- Applications
- WB, IHC, IF, ELISA
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- WB 1:500-1:2000. IHC 1:200-1:1000. IF 1:200-1:1000. ELISA 1:10000.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MDM4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3206z | Mouse Anti-MDM4 Recombinant Antibody (clone 18A4) | WB, ICC, IF, IHC, ELISA | Mouse IgG1 |
| MOB-1013CT | Recombinant Mouse anti-Human MDM4 Monoclonal antibody (3E20G5) | ELISA, ICC, IF, IHC-P, WB | |
| VS3-QX763 | Mouse Anti-MDM4 Recombinant Antibody (clone 2D10F4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-0425-WR375 | Mouse Anti-MDM4 Monoclonal Antibody (VS7-0425-WR375) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-097 | Recombinant Anti-MDM4 VHH Single Domain Antibody | Inhibition | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY162 | CytoStream™ Mouse Anti-MDM4 Recombinant Antibody (clone QCRL-3) | FC | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4324 | Anti-MDM4 Immunohistochemistry Kit | IHC |
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