Recombinant Humanized Anti-MERS-CoV Antibody (m336)
CAT#: HPAB-2420LY
This product is an antibody clone m336 targeting MERS-CoV. The antibody m336 is recommended for immunoassay techniques including ELISA.
Published Data
Neut
Figure 1 Viral titers in rabbit lungs following intravenous prophylaxis with neutralizing monoclonal antibody.
Rabbits received Middle East respiratory syndrome coronavirus (MERS-CoV)-specific human monoclonal antibody (hmAb) m336 or irrelevant control hmAb m102.4 intravenously 24 hours before intranasal infection with 105 50% tissue culture infective doses (TCID50) of EMC/2012. A, Lungs were collected on days 1 and 3 after infection, and viral titers were quantified by quantitative real time polymerase chain reaction analysis. **P <.005 and ***P <.001 by 1-way analysis of variance, with the Tukey multiple comparisons test. Abbreviation: eq, equivalents.
Houser, K. V., Gretebeck, L., Ying, T., Wang, Y., Vogel, L., Lamirande, E. W.,... & Subbarao, K. (2016). Prophylaxis with a Middle East respiratory syndrome coronavirus (MERS-CoV)-specific human monoclonal antibody protects rabbits from MERS-CoV infection. The Journal of infectious diseases, 213(10), 1557-1561.
Neut
Figure 2 Viral titers in rabbit lungs following intranasal prophylaxis with neutralizing monoclonal antibody.
Rabbits received Middle East respiratory syndrome coronavirus (MERS-CoV)-specific human monoclonal antibody (hmAb) m336 or irrelevant control hmAb m102.4 intranasally 24 hours before intranasal infection with 105 50% tissue culture infective doses (TCID50) of EMC/2012. A, Lungs were collected on days 1 and 3 after infection, and viral titers were quantified by quantitative real time polymerase chain reaction analysis. **P <.005 and ***P <.001 by 1-way analysis of variance, with the Tukey multiple comparisons test. Abbreviation: eq, equivalents.
Houser, K. V., Gretebeck, L., Ying, T., Wang, Y., Vogel, L., Lamirande, E. W.,... & Subbarao, K. (2016). Prophylaxis with a Middle East respiratory syndrome coronavirus (MERS-CoV)-specific human monoclonal antibody protects rabbits from MERS-CoV infection. The Journal of infectious diseases, 213(10), 1557-1561.
Neut
Figure 3 Strong synergism of HR2P-M2 combined with m336 against MERS-CoV pseudovirus infection.
The effective concentrations for inhibiting MERS-CoV pseudovirus infection are plotted in two curves. The blue curves represent inhibitors used alone, and the red curves represent each inhibitor used in combination. The width between two curves represents the fold of enhancement between an inhibitor used alone and in combination.
Wang, C., Hua, C., Xia, S., Li, W., Lu, L., & Jiang, S. (2019). Combining a fusion inhibitory peptide targeting the MERS-CoV S2 protein HR1 domain and a neutralizing antibody specific for the S1 protein receptor-binding domain (RBD) showed potent synergism against pseudotyped MERS-CoV with or without mutations in RBD. Viruses, 11(1), 31.
FuncS
Figure 4 Strong synergism resulting from the HR2P-M2/m336 combination against MERS-CoV S protein-mediated cell–cell fusion.
Wang, C., Hua, C., Xia, S., Li, W., Lu, L., & Jiang, S. (2019). Combining a fusion inhibitory peptide targeting the MERS-CoV S2 protein HR1 domain and a neutralizing antibody specific for the S1 protein receptor-binding domain (RBD) showed potent synergism against pseudotyped MERS-CoV with or without mutations in RBD. Viruses, 11(1), 31.
FuncS
Figure 5 MERS-CoV pseudovirus inhibitory activity as determined from sera of BALB/c mice treated with m336 (0.01 mg) alone, HR2P-M2 (1 mg) alone, or m336 (0.01 mg)/HR2P-M2 (1 mg) in combination.
Data are presented as means ± SD. **, and *** represent p < 0.01, and p < 0.001, respectively.
Wang, C., Hua, C., Xia, S., Li, W., Lu, L., & Jiang, S. (2019). Combining a fusion inhibitory peptide targeting the MERS-CoV S2 protein HR1 domain and a neutralizing antibody specific for the S1 protein receptor-binding domain (RBD) showed potent synergism against pseudotyped MERS-CoV with or without mutations in RBD. Viruses, 11(1), 31.
ELISA
Figure 6 Binding of IgG1s m336, m336-gH, m336-gL-FR, m336-gL and a negative control IgG1 m102.4 to MERS-CoV S1 protein measured by ELISA.
Ying, T., Prabakaran, P., Du, L., Shi, W., Feng, Y., Wang, Y.,... & Zhou, T. (2015). Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody. Nature communications, 6(1), 1-10.
Neut
Figure 7 Neutralization of viruses pseudotyped with the MERS-CoV S glycoprotein.
Ying, T., Prabakaran, P., Du, L., Shi, W., Feng, Y., Wang, Y.,... & Zhou, T. (2015). Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody. Nature communications, 6(1), 1-10.
ELISA
Figure 8 Binding of Fab m336 allele-specific mutants (F54L and K73E) to MERS-CoV S1 protein measured by ELISA.
Ying, T., Prabakaran, P., Du, L., Shi, W., Feng, Y., Wang, Y.,... & Zhou, T. (2015). Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody. Nature communications, 6(1), 1-10.
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Specifications
- Host Species
- Humanized
- Specificity
- MERS-CoV
- Species Reactivity
- MERS-CoV
- Clone
- m336
- Applications
- ELISA, Neut, FuncS
Product Property
- Purification
- 95% pure by SDS-PAGE
- Purity
- > 95% Purity determined by SDS-PAGE.
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
Applications
- Application Notes
- Enzyme-Linked Immunosorbent Assay
Binding of antibodies to the MERS-CoV S1 protein was measured by ELISA. Serial fivefold dilutions of antibodies starting from 1 μM were diluted in PBS and assayed for binding to MERS-CoV S1 protein. After washing, bound antibodies were detected by horseradish peroxidase (HRP)-conjugated goat anti-human IgG Ab or horseradish peroxidase-conjugated anti-FLAG Ab.
Neutralization Assay
For measuring the neutralization activities of m336, m336-gH, m336-gL-FR and m336-gL, Huh-7 cells were infected with MERS-CoV-S pseudoviruses in the presence of serial dilutions of antibodies at indicated concentrations. The culture was re-fed with fresh medium 12 h post infection and incubated for an additional 72 h. Cells were washed with PBS and lysed using lysis reagent included in a luciferase kit. Aliquots of cell lysates were transferred to 96-well flat-bottom luminometer plates (Costar), followed by addition of luciferase substrate. Relative light units were determined immediately using the Ultra 384 luminometer.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Lot Number:
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- CAT
- Product Name
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| MOR-4106 | Hi-Affi™ Recombinant Rabbit Anti-MERS-CoV Monoclonal Antibody (SI177DS) | ELISA, IHC-P, ICC, IF, IF, FC | IgG |
| MOR-4453 | Hi-Affi™ Recombinant Rabbit Anti-MERS-CoV Monoclonal Antibody (SI546DS) | MN | IgG |
| CAT | Product Name | Application | Type |
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| MOB-010CQ | Rabbit Anti-MERS-CoV Antibody | Neut | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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| HPAB-0921LY-S(P) | Mouse Anti-MERS-CoV Recombinant Antibody; scFv Fragment (HPAB-0921LY-S(P)) | ELISA | Mouse scFv |
| HPAB-0922LY-S(P) | Mouse Anti-MERS-CoV Recombinant Antibody; scFv Fragment (HPAB-0922LY-S(P)) | ELISA | Mouse scFv |
| HPAB-0923LY-S(P) | Mouse Anti-MERS-CoV Recombinant Antibody; scFv Fragment (HPAB-0923LY-S(P)) | ELISA | Mouse scFv |
| HPAB-0924LY-S(P) | Mouse Anti-MERS-CoV Recombinant Antibody; scFv Fragment (HPAB-0924LY-S(P)) | ELISA | Mouse scFv |
| HPAB-0925LY-S(P) | Mouse Anti-MERS-CoV Recombinant Antibody; scFv Fragment (HPAB-0925LY-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0921LY-F(E) | Mouse Anti-MERS-CoV Recombinant Antibody; Fab Fragment (HPAB-0921LY-F(E)) | ELISA | Mouse Fab |
| HPAB-0922LY-F(E) | Mouse Anti-MERS-CoV Recombinant Antibody; Fab Fragment (HPAB-0922LY-F(E)) | ELISA | Mouse Fab |
| HPAB-0923LY-F(E) | Mouse Anti-MERS-CoV Recombinant Antibody; Fab Fragment (HPAB-0923LY-F(E)) | ELISA | Mouse Fab |
| HPAB-2420LY-F(E) | Humanized Anti-MERS-CoV Antibody Fab Fragment (m336) | ELISA | |
| HPAB-M0144-YC-F(E) | Human Anti-MERS-Cov Recombinant Antibody Fab Fragment (HPAB-M0144-YC-F(E)) | ELISA, Neut | Humanized Fab |
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